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Literature summary extracted from

  • Hao, J.; Vann, W.F.; Hinderlich, S.; Sundaramoorthy, M.
    Elimination of 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity from human N-acetylneuraminic acid 9-phosphate synthase by a single mutation (2006), Biochem. J., 397, 195-201.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.57 expressed in Escherichia coli Homo sapiens
2.5.1.132 expressed in Escherichia coli BL21(DE3) Gold cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.57 M42L mutant shows the additional 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity like the wild type enzyme Homo sapiens
2.5.1.57 M42T the mutation abolishes the 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity completely without compromising the Neu5Ac-9-P synthase activity Homo sapiens
2.5.1.132 M42L the M42L mutant of N-acetylneuraminic acid-9-phosphate synthase, like the wild type enzyme, shows the additional 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity Homo sapiens
2.5.1.132 M42T the mutation of N-acetylneuraminic acid-9-phosphate synthase abolishes its 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity Homo sapiens
2.5.1.132 R96K/R100S/E103Q/V105I/V195A the mutant of N-acetylneuraminic acid-9-phosphate synthase has significant 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.57 1.04
-
 N-acetyl-D-mannosamine 6-phosphate wild type enzyme, at 37°C Homo sapiens
2.5.1.57 1.26
-
 N-acetyl-D-mannosamine 6-phosphate mutant enzyme M42T, at 37°C Homo sapiens
2.5.1.57 1.72
-
 N-acetyl-D-mannosamine 6-phosphate mutant enzyme M42L, at 37°C Homo sapiens
2.5.1.57 2.5 5 D-mannose 6-phosphate mutant enzyme M42L, at 37°C Homo sapiens
2.5.1.57 2.62
-
 D-mannose 6-phosphate wild type enzyme, at 37°C Homo sapiens
2.5.1.132 2.5 5 D-mannose 6-phosphate mutant enzyme M42L, at pH 7.5 and 37°C Homo sapiens
2.5.1.132 2.62
-
 D-mannose 6-phosphate wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.57 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.132 phosphoenolpyruvate + D-mannose 6-phosphate + H2O Homo sapiens
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.57 Homo sapiens
-
-
-
2.5.1.132 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.57 Ni2+ chelating Sepharose column chromatography Homo sapiens
2.5.1.132 nickel affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.57 D-mannose 6-phosphate + phosphoenolpyruvate + H2O
-
 Homo sapiens 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Homo sapiens N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.132 phosphoenolpyruvate + D-mannose 6-phosphate + H2O
-
 Homo sapiens 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.57 N-acetylneuraminic acid 9-phosphate synthase
-
 Homo sapiens
2.5.1.57 Neu5Ac 9-phosphate synthase
-
 Homo sapiens
2.5.1.132 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase
-
 Homo sapiens
2.5.1.132 Neu5Ac 9-phosphate synthase shows also -keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity Homo sapiens
2.5.1.132 neu5ac-9-P synthase shows also -keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.57 0.008
-
 D-mannose 6-phosphate mutant enzyme M42L, at 37°C Homo sapiens
2.5.1.57 0.011
-
 D-mannose 6-phosphate wild type enzyme, at 37°C Homo sapiens
2.5.1.57 0.013
-
 N-acetyl-D-mannosamine 6-phosphate mutant enzyme M42T, at 37°C Homo sapiens
2.5.1.57 0.016
-
 N-acetyl-D-mannosamine 6-phosphate mutant enzyme M42L, at 37°C Homo sapiens
2.5.1.57 0.022
-
 N-acetyl-D-mannosamine 6-phosphate wild type enzyme, at 37°C Homo sapiens
2.5.1.132 0.0011
-
 D-mannose 6-phosphate wild type enzyme, at pH 7.5 and 37°C Homo sapiens
2.5.1.132 0.0083
-
 D-mannose 6-phosphate mutant enzyme M42L, at pH 7.5 and 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.132 0.000327
-
 D-mannose 6-phosphate mutant enzyme M42L, at pH 7.5 and 37°C Homo sapiens
2.5.1.132 0.000426
-
 D-mannose 6-phosphate wild type enzyme, at pH 7.5 and 37°C Homo sapiens