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Literature summary extracted from

  • Brige, A.; Van den Hemel, D.; Carpentier, W.; De Smet, L.; Van Beeumen, J.J.
    Comparative characterization and expression analysis of the four Old Yellow Enzyme homologues from Shewanella oneidensis indicate differences in physiological function (2006), Biochem. J., 394, 335-344.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.99.1 expression in Escherichia coli Shewanella oneidensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.99.1 0.0016
-
 NEM pH 7.3 Shewanella oneidensis
1.6.99.1 0.0264
-
 nitroglycerin pH 7.3 Shewanella oneidensis
1.6.99.1 0.0547
-
 2-cyclohexen-1-one pH 7.3 Shewanella oneidensis
1.6.99.1 0.109
-
 acrolein pH 7.3 Shewanella oneidensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.99.1 NADPH + H+ + 2-cyclohexen-1-one Shewanella oneidensis the substrate results in a very rapid and strong reduction of SYE4 NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + acrolein Shewanella oneidensis the substrate results in a very rapid and strong reduction of SYE4 NADP+ + propionaldehyde
-
 ?
1.6.99.1 NADPH + H+ + NEM Shewanella oneidensis the substrate results in a very rapid and strong reduction of SYE4 NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + nitroglycerin Shewanella oneidensis the substrate results in a very rapid and strong reduction of SYE4 NADP+ + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.99.1 Shewanella oneidensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.99.1 recombinant glutathione-S-transferase-tagged enzyme Shewanella oneidensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.99.1 NADPH + H+ + 2-cyclohexen-1-one the substrate results in a very rapid and strong reduction of SYE4 Shewanella oneidensis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + acrolein the substrate results in a very rapid and strong reduction of SYE4 Shewanella oneidensis NADP+ + propionaldehyde
-
 ?
1.6.99.1 NADPH + H+ + NEM the substrate results in a very rapid and strong reduction of SYE4 Shewanella oneidensis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + nitroglycerin the substrate results in a very rapid and strong reduction of SYE4 Shewanella oneidensis NADP+ + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.99.1 SYE4
-
 Shewanella oneidensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.99.1 0.03 0.55 nitroglycerin pH 7.3 Shewanella oneidensis
1.6.99.1 0.052 2.1 NEM pH 7.3 Shewanella oneidensis
1.6.99.1 0.41
-
 acrolein pH 7.3 Shewanella oneidensis
1.6.99.1 4.75
-
 2-cyclohexen-1-one pH 7.3 Shewanella oneidensis
1.6.99.1 6.84
-
 nitroglycerin pH 7.3 Shewanella oneidensis
1.6.99.1 9.54
-
 NEM pH 7.3 Shewanella oneidensis
1.6.99.1 11.66
-
 acrolein pH 7.3 Shewanella oneidensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.99.1 NADPH
-
 Shewanella oneidensis