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Literature summary extracted from

  • Shokeer, A.; Larsson, A.K.; Mannervik, B.
    Residue 234 in glutathione transferase T1-1 plays a pivotal role in the catalytic activity and the selectivity against alternative substrates (2005), Biochem. J., 388, 387-392.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expression of wild-type and mutant N-terminally His6-tagged isozymes GST T1-1 in Escherichia coli strain XL-1 blue Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 W234K site-directed mutagenesis, isozyme GST T1-1, the mutant shows altered substrate specificity compared to the wild-type enzyme Homo sapiens
2.5.1.18 W234R site-directed mutagenesis, isozyme GST T1-1, amino acid exchange analogously to the murine enzyme, the mutant shows altered substrate specificity compared to the wild-type enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Homo sapiens
-
isozyme GST T1-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant wild-type and mutant N-terminally His6-tagged isozymes GST T1-1 from Escherichia coli strain XL-1 blue by nickel affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 additional information
-
substrate specificity of wild-type and mutant isozymes GST T1-1, overview Homo sapiens
2.5.1.18 4.96
-
purified wild-type GST T1-1, substrate 1-iodomethane Homo sapiens
2.5.1.18 7.84
-
purified wild-type GST T1-1, substrate 1,2-epoxy-3-(4-nitro-phenoxy)propane Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-epoxy-3-(4-nitro-phenoxy)propane best substrate for the wild-type GST T1-1 and mutant W234K Homo sapiens ?
-
?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
Homo sapiens chloride + 2,4-dinitrophenyl-glutathione
-
?
2.5.1.18 glutathione + 1-iodo-3-phenylpropane
-
Homo sapiens iodide + 3-phenylpropyl-glutathione
-
?
2.5.1.18 glutathione + 1-iodobutane
-
Homo sapiens iodide + butyl-glutathione
-
?
2.5.1.18 glutathione + 1-iodoethane
-
Homo sapiens iodide + ethylglutathione
-
?
2.5.1.18 glutathione + 1-iodoheptane
-
Homo sapiens iodide + heptyl-glutathione
-
?
2.5.1.18 glutathione + 1-iodohexane
-
Homo sapiens iodide + hexyl-glutathione
-
?
2.5.1.18 glutathione + 1-iodomethane best substrate of GST T1-1 mutant W234R Homo sapiens iodide + methylglutathione
-
?
2.5.1.18 glutathione + 1-iodooctane
-
Homo sapiens iodide + octyl-glutathione
-
?
2.5.1.18 glutathione + 1-iodopentane
-
Homo sapiens iodide + pentyl-glutathione
-
?
2.5.1.18 glutathione + 1-iodopropane
-
Homo sapiens iodide + propyl-glutathione
-
?
2.5.1.18 glutathione + 1-menaphthyl sulfate
-
Homo sapiens ?
-
?
2.5.1.18 glutathione + 4-nitrobenzyl chloride
-
Homo sapiens chloride + 4-nitrobenzyl-glutathione
-
?
2.5.1.18 glutathione + 4-nitrophenethyl bromide
-
Homo sapiens bromide + 4-nitrophenethyl-glutathione
-
?
2.5.1.18 glutathione + cumene hydroperoxide
-
Homo sapiens ?
-
?
2.5.1.18 glutathione + styrene 7,8-oxide
-
Homo sapiens ?
-
?
2.5.1.18 additional information substrate specificity of wild-type and mutant isozymes GST T1-1, overview Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GST
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 25 30 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 8
-
assay at Homo sapiens