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Literature summary extracted from

  • Ito, S.; Hamada, S.; Yamaguchi, K.; Umene, S.; Ito, H.; Matsui, H.; Ozawa, T.; Taguchi, H.; Watanabe, J.; Wasaki, J.; Ito, S.
    Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus (2007), Biochem. Biophys. Res. Commun., 360, 640-645.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.11 expressed in Escherichia coli Ruminococcus albus

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.11 additional information sequence analysis shows that the gene shares a very low level of homology with N-acetyl-D-glucosamine 2-epimerases (EC 5.1.3.8), but no significant homology to any other epimerases reported to date Ruminococcus albus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.11 43100
-
 SDS-PAGE Ruminococcus albus

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.11 Ruminococcus albus P0DKY4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.11 protein is purified by loading the extract directly onto coupled columns (15 x 150 mm each) of CM Sepharose Fast Flow and DEAE Sepharose CL-6B Ruminococcus albus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.11 3
-
 the specific activity toward cellobiose is 3–5 micromol/min/mg at a pH optimum 7.8 in 100 mM glycylglycine buffer Ruminococcus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.11 cellobiose the enzyme is found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose Ruminococcus albus 4-O-beta-D-glucopyranosyl-D-mannose
-
 ?
5.1.3.11 cellotetraose the enzyme is found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose Ruminococcus albus 4-O-beta-D-glucopyranosyl-(1-4)-O-beta-D-glucopyranosyl-(1-4)-O-beta-D-glucopyranosyl-(1-4)-D-mannose
-
 ?
5.1.3.11 cellotriose the enzyme is found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose Ruminococcus albus 4-O-beta-D-glucopyranosyl-(1-4)-O-beta-D-glucopyranosyl-(1-4)-D-mannose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.3.11 CE-NE1
-
 Ruminococcus albus
5.1.3.11 cellobiose 2-epimerase
-
 Ruminococcus albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.11 30
-
 assay at Ruminococcus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.11 7.8
-
-
 Ruminococcus albus