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Literature summary extracted from

  • Sasaki, N.; Yoshida, H.; Fuwa, T.J.; Kinoshita-Toyoda, A.; Toyoda, H.; Hirabayashi, Y.; Ishida, H.; Ueda, R.; Nishihara, S.
    Drosophila beta1,4-N-acetylgalactosaminyltransferase-A synthesizes the LacdiNAc structures on several glycoproteins and glycosphingolipids (2007), Biochem. Biophys. Res. Commun., 354, 522-527.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.92 enzyme expression analysis in larvae, expression of the FLAG-tagged catalytic domain, comprisisng residues 62–403, in the Spodoptera frugiperda Sf21 cell baculovirus expression system Drosophila melanogaster
2.4.1.244 expressed in sf21 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.92 additional information enzyme activity in beta4GalNAcT RNAi flies is reduced Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.92 Mn2+
-
 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.92 additional information Drosophila melanogaster the enzyme synthesizes the LacdiNAc structures, with GalNAcbeta1,4GlcNAc, on several glycoproteins and glycosphingolipids, including N-glycans, O-glycans, and the arthro-series GSLs, GalNAcbeta1,4Glc-NAcbeta1,3Manbeta1,4Glcbeta1Cer, and neutral GSLs, in vitro and in vivo, the GalNAcbeta1,4GlcNAc, i.e. LacdiNAc or LDN, structure is a more common structural feature in invertebrate glycoconjugates when compared with the Galbeta1,4GlcNAc, i.e. LacNAc or LN, structure ?
-
 ?
2.4.1.244 UDP-N-acetyl-D-galactosamine + N-acetyl-beta-D-glucosaminyl-R group Drosophila sp. (in: flies) beta4GalNAcTA contributs to the synthesis of the GalNAcbeta1,4GlcNA structure in vivo and may play a role in the physiological or neuronal development of Drosophila UDP + N-acetyl-beta-D-galactosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl-R group
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.92 Drosophila melanogaster
-
-
-
2.4.1.244 Drosophila melanogaster
-
-
-
2.4.1.244 Drosophila sp. (in: flies)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.92 recombinant FLAG-tagged catalytic domain from Spodoptera frugiperda Sf21 cells by anti-FLAG M1 affinity chromatography Drosophila melanogaster
2.4.1.244 anti-FLAG M1 affinity gel filtration Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.92 larva third instar larvae Drosophila melanogaster
-
2.4.1.244 embryo beta4GalNAcT mRNA is expressed throughout the life cycle of the fly, i.e., from the embryo stage. In the larval stage, mRNA is expressed to a greater extent in the eye disc and larval brain than in the wing disc and leg disc Drosophila sp. (in: flies)
-
2.4.1.244 larva beta4GalNAcT mRNA is expressed throughout the life cycle of the fly, i.e., from the embryo stage. In the larval stage, mRNA is expressed to a greater extent in the eye disc and larval brain than in the wing disc and leg disc Drosophila sp. (in: flies)
-
2.4.1.244 neuron highly expressed in the developmental and adult neuronal tissues Drosophila sp. (in: flies)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.92 additional information
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.92 additional information the enzyme synthesizes the LacdiNAc structures, with GalNAcbeta1,4GlcNAc, on several glycoproteins and glycosphingolipids, including N-glycans, O-glycans, and the arthro-series GSLs, GalNAcbeta1,4Glc-NAcbeta1,3Manbeta1,4Glcbeta1Cer, and neutral GSLs, in vitro and in vivo, the GalNAcbeta1,4GlcNAc, i.e. LacdiNAc or LDN, structure is a more common structural feature in invertebrate glycoconjugates when compared with the Galbeta1,4GlcNAc, i.e. LacNAc or LN, structure Drosophila melanogaster ?
-
 ?
2.4.1.92 additional information no activity with donor UDP-galactose, the enzyme synthesizes the LacdiNAc structures, with GalNAcbeta1,4GlcNAc, on several glycoproteins and glycosphingolipids, including N-glycans, O-glycans, and the arthro-series GSLs, such as GalNAcbeta1,4Glc-NAcbeta1,3Manbeta1,4Glcbeta1Cer, and neutral GSLs, in vitro and in vivo Drosophila melanogaster ?
-
 ?
2.4.1.92 UDP-GalNAc + GlcNAcbeta1,3Fuc DNS-labeled acceptor substrate Drosophila melanogaster UDP + GalNAcbeta1,4GlcNAcbeta1,3Fuc
-
 ?
2.4.1.244 additional information no activity with GalNac Drosophila melanogaster ?
-
 ?
2.4.1.244 UDP-GalNAc + GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-[Fuc-alpha-(1,6)]-GlcNAc-2-pyridylaminoside
-
 Drosophila melanogaster UDP + GalNAc-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-[Fuc-alpha-(1,6)]-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
-
 ?
2.4.1.244 UDP-GalNAc + GlcNAc-beta-(1,3)-GalNAc-alpha-p-nitrophenyl
-
 Drosophila melanogaster UDP + GalNAc-beta-(1,4)-GlcNAc-beta-(1,3)-GalNAc-alpha-p-nitrophenyl
-
 ?
2.4.1.244 UDP-GalNAc + GlcNAc-beta-(1,3)Fuc-dansyl
-
 Drosophila melanogaster UDP + GalNAc-beta-(1,4)-GlcNAc-beta-(1,3)Fuc-dansyl
-
 ?
2.4.1.244 UDP-N-acetyl-D-galactosamine + GlcNAcbeta1,2Manalpha1,6[GlcNAcbeta1,2Manalpha1,3]Manbeta1,4GlcNAcbeta1,4GlcNAc-aminopyridine
-
 Drosophila sp. (in: flies) ?
-
 ?
2.4.1.244 UDP-N-acetyl-D-galactosamine + GlcNAcbeta1,2Manalpha1,6[GlcNAcbeta1,4[GlcNAcbeta1,2]Manalpha1,3]Manbeta1,4GlcNAcbeta1,4GlcNAc-aminopyridine
-
 Drosophila sp. (in: flies) ?
-
 ?
2.4.1.244 UDP-N-acetyl-D-galactosamine + GlcNAcbeta1,3Fuc-dansyl GalNAc is efficiently transferred toward N-glycans, O-glycans, and the arthro-series glycosphingolipids Drosophila sp. (in: flies) ?
-
 ?
2.4.1.244 UDP-N-acetyl-D-galactosamine + N-acetyl-beta-D-glucosaminyl-R group beta4GalNAcTA contributs to the synthesis of the GalNAcbeta1,4GlcNA structure in vivo and may play a role in the physiological or neuronal development of Drosophila Drosophila sp. (in: flies) UDP + N-acetyl-beta-D-galactosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl-R group
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.92 beta 1,4-N-acetylgalactosaminyltransferase-A
-
 Drosophila melanogaster
2.4.1.92 beta4GalNAcT
-
 Drosophila melanogaster
2.4.1.244 beta 1,4-N-acetylgalactosaminyltransferase-A
-
 Drosophila melanogaster
2.4.1.244 beta1,4-N-acetylgalactosaminyltransferase
-
 Drosophila sp. (in: flies)
2.4.1.244 beta4GalNAcT
-
 Drosophila melanogaster
2.4.1.244 beta4GalNAcT
-
 Drosophila sp. (in: flies)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.92 25
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.92 7.2 7.4 assay at Drosophila melanogaster