EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.16 | Cymal 5 | optimal concentration is 0.002%, decrease in activity above 0.05% | Bos taurus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.16 | additional information | replacement of N-terminal membrane anchor and basic regions by the basic regions of CYP2C3 for efficient expression and purification. N-terminal membrane anchor and sequence of the basic region do not significantly affect either substrate-specificity or 21-hydroxylase activites | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.16 | 0.0108 | - |
17alpha-hydroxyprogesterone | 37°C, pH 7.4, presence of 0.002% Cymal 5 | Bos taurus | |
1.14.14.16 | 0.0129 | - |
progesterone | 37°C, pH 7.4, presence of 0.002% Cymal 5 | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.16 | 12400 | - |
gel filtration in presence of 0.12% Cymal 5, protein elutes as monomer surrounded by a micelle of detergent | Bos taurus |
1.14.14.16 | 53000 | - |
1 * 53000, calculated | Bos taurus |
1.14.14.16 | 80700 | - |
gel filtration in presence of 1% cholate, protein elutes as monomer surrounded by a micelle of cholate | Bos taurus |
1.14.14.16 | 167000 | - |
gel filtration in presence of 0.056% Cymal 6, protein elutes as monomer surrounded by a micelle of detergent | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.16 | Bos taurus | P00191 | expressed in Escherichia coli | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.16 | 17alpha-hydroxyprogesterone + NADPH + O2 | - |
Bos taurus | 17,21-dihydroxyprogesterone + NADP+ + H2O | - |
? | |
1.14.14.16 | progesterone + NADPH + O2 | - |
Bos taurus | deoxycorticosterone + NADP+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.16 | monomer | 1 * 53000, calculated | Bos taurus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.16 | P450c21 | - |
Bos taurus |