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Literature summary extracted from

  • Arase, M.; Waterman, M.R.; Kagawa, N.
    Purification and characterization of bovine steroid 21-hydroxylase (P450c21) efficiently expressed in Escherichia coli (2006), Biochem. Biophys. Res. Commun., 344, 400-405.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.16 Cymal 5 optimal concentration is 0.002%, decrease in activity above 0.05% Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.16 additional information replacement of N-terminal membrane anchor and basic regions by the basic regions of CYP2C3 for efficient expression and purification. N-terminal membrane anchor and sequence of the basic region do not significantly affect either substrate-specificity or 21-hydroxylase activites Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.16 0.0108
-
17alpha-hydroxyprogesterone 37°C, pH 7.4, presence of 0.002% Cymal 5 Bos taurus
1.14.14.16 0.0129
-
progesterone 37°C, pH 7.4, presence of 0.002% Cymal 5 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.16 12400
-
gel filtration in presence of 0.12% Cymal 5, protein elutes as monomer surrounded by a micelle of detergent Bos taurus
1.14.14.16 53000
-
1 * 53000, calculated Bos taurus
1.14.14.16 80700
-
gel filtration in presence of 1% cholate, protein elutes as monomer surrounded by a micelle of cholate Bos taurus
1.14.14.16 167000
-
gel filtration in presence of 0.056% Cymal 6, protein elutes as monomer surrounded by a micelle of detergent Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.16 Bos taurus P00191 expressed in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.16 17alpha-hydroxyprogesterone + NADPH + O2
-
Bos taurus 17,21-dihydroxyprogesterone + NADP+ + H2O
-
?
1.14.14.16 progesterone + NADPH + O2
-
Bos taurus deoxycorticosterone + NADP+ + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.14.16 monomer 1 * 53000, calculated Bos taurus

Synonyms

EC Number Synonyms Comment Organism
1.14.14.16 P450c21
-
Bos taurus