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Literature summary extracted from

  • Sueda, S.; Li, Y.Q.; Kondo, H.; Kawarabayasi, Y.
    Substrate specificity of archaeon Sulfolobus tokodaii biotin protein ligase (2006), Biochem. Biophys. Res. Commun., 344, 155-159.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.15 expression in Escherichia coli BL21(DE3) Sulfurisphaera tokodaii

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.15 A43G catalytic efficiency is 2fold lower than catalytic efficiency of wild-type enzyme Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.15 ATP
-
 Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.15 0.000026
-
 biotin pH 8.0, 37°C, mutant enzyme A43G Sulfurisphaera tokodaii
6.3.4.15 0.00044
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine pH 8.0, 70°C, wild type enzyme Sulfurisphaera tokodaii
6.3.4.15 0.00058
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine pH 8.0, 70°C, mutant enzyme A43G Sulfurisphaera tokodaii
6.3.4.15 0.018
-
 ATP pH 8.0, 37°C, mutant enzyme A43G Sulfurisphaera tokodaii
6.3.4.15 0.063
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine pH 8.0, 70°C, mutant enzyme A43G Sulfurisphaera tokodaii
6.3.4.15 0.101
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine pH 8.0, 70°C, wild type enzyme Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Sulfurisphaera tokodaii F9VNG7
-
-
6.3.4.15 Sulfurisphaera tokodaii 7 F9VNG7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate Sulfurisphaera tokodaii AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine
-
 ?
6.3.4.15 ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate Sulfurisphaera tokodaii 7 AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine
-
 ?
6.3.4.15 ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate Sulfurisphaera tokodaii AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine
-
 ?
6.3.4.15 ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate Sulfurisphaera tokodaii 7 AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.15 More biotin-protein-ligase/holo-biotin-carboxyl-carrier-protein complex Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 STK_15250 locus name Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.15 80
-
 no denaturation Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.15 0.0022
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine pH 8.0, 70°C, mutant enzyme A43G Sulfurisphaera tokodaii
6.3.4.15 0.004
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine pH 8.0, 70°C, wild type enzyme Sulfurisphaera tokodaii
6.3.4.15 0.082
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine pH 8.0, 70°C, mutant enzyme A43G Sulfurisphaera tokodaii
6.3.4.15 0.165
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine pH 8.0, 70°C, wild type enzyme Sulfurisphaera tokodaii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.4.15 0.36
-
 ATP pH 8.0, 37°C, mutant enzyme A43G Sulfurisphaera tokodaii
6.3.4.15 0.62
-
 ATP pH 8.0, 37°C, wild type enzyme Sulfurisphaera tokodaii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.4.15 1.3
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine pH 8.0, 70°C, mutant enzyme A43G Sulfurisphaera tokodaii
6.3.4.15 1.6
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine pH 8.0, 70°C, wild type enzyme Sulfurisphaera tokodaii
6.3.4.15 3.8
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine pH 8.0, 70°C, mutant enzyme A43G Sulfurisphaera tokodaii
6.3.4.15 9.1
-
 [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine pH 8.0, 70°C,wild type enzyme Sulfurisphaera tokodaii