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Literature summary extracted from
- The unique glutathione reductase from Xanthomonas campestris: gene expression and enzyme characterization (2005), Biochem. Biophys. Res. Commun., 331, 1324-1330.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | expressed in Escherichia coli | Xanthomonas campestris |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.7 | 0.0526 | - |
NADPH | - |
Xanthomonas campestris |  |
| 1.8.1.7 | 0.0555 | - |
NADH | - |
Xanthomonas campestris | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 50000 | - |
SDS-PAGE | Xanthomonas campestris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Xanthomonas campestris | Q56S00 | Xanthomonas campestris pv. phaseoli | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | nickel affinity column chromatography | Xanthomonas campestris |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 0.039 | - |
using NADH as electron donor | Xanthomonas campestris |
| 1.8.1.7 | 0.045 | - |
using NADPH as electron donor | Xanthomonas campestris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | glutathione disulfide + NADH + H+ | - |
Xanthomonas campestris | 2 glutathione + NAD+ | - |
? | |
| 1.8.1.7 | glutathione disulfide + NADH + H+ | - |
Xanthomonas campestris | glutathione + NAD+ | - |
? | |
| 1.8.1.7 | glutathione disulfide + NADPH + H+ | - |
Xanthomonas campestris | glutathione + NADP+ | - |
r | |
| 1.8.1.7 | additional information | the enzyme has the ability to utilize both NADPH and NADH as electron donors | Xanthomonas campestris | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | monomer | 1 * 50000, gel filtration | Xanthomonas campestris |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | glutathione reductase | - |
Xanthomonas campestris |
| 1.8.1.7 | Gor | - |
Xanthomonas campestris |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.7 | 32.5 | - |
NADH | - |
Xanthomonas campestris | |
| 1.8.1.7 | 37.5 | - |
NADPH | - |
Xanthomonas campestris | |
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Release 2023.1 (January 2023)
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