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Literature summary extracted from

  • Tsai, S.C.; Li, Y.K.
    Purification and characterization of a catechol 1,2-dioxygenase from a phenol degrading Candida albicans TL3 (2007), Arch. Microbiol., 187, 199-206.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 AgNO3 0.001 mM, 93% loss of activity Candida albicans
1.13.11.1 CuCl2 0.1 mM, 41% inhibition Candida albicans
1.13.11.1 FeCl3 0.1 mM, 14% inhibition Candida albicans
1.13.11.1 HgCl2 0.1 mM, 64% inhibition Candida albicans
1.13.11.1 PbSO4 0.1 mM, 75% loss of activity Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.0093
-
 catechol 25°C Candida albicans
1.13.11.1 0.0215
-
 4-methylcatechol 25°C Candida albicans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Fe each subunit contained one iron Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 32000
-
 2 * 32000, SDS-PAGE Candida albicans
1.13.11.1 64000
-
 gel filtration Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Candida albicans
-
-
-
1.13.11.1 Candida albicans TL3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 63
-
-
 Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 4-methylcatechol + O2
-
 Candida albicans 3-methyl-cis,cis-muconate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Candida albicans TL3 3-methyl-cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Candida albicans cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Candida albicans TL3 cis,cis-muconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 dimer 2 * 32000, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 1,2-CTD
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 25
-
-
 Candida albicans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.1 40
-
 pH 8.3, 30 min, stable below Candida albicans
1.13.11.1 50
-
 pH 8.3, 30 min, about 35% of maximal activity Candida albicans
1.13.11.1 60
-
 pH 8.3, 30 min, about 85% of maximal activity Candida albicans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.1 2 8 catechol 25°C Candida albicans
1.13.11.1 5.6
-
 4-methylcatechol 25°C Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 8
-
-
 Candida albicans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 6 9.5 pH 6.0: about 60% of maximal activity, pH 9.5: about 60% of maximal activity Candida albicans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.1 7 9 30 min, maintaining more than 85% activity Candida albicans