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Literature summary extracted from

  • Genestra, M.; Guedes-Silva, D.; Souza, W.J.; Cysne-Finkelstein, L.; Soares-Bezerra, R.J.; Monteiro, F.P.; Leon, L.L.
    Nitric oxide synthase (NOS) characterization in Leishmania amazonensis axenic amastigotes (2006), Arch. Med. Res., 37, 328-333.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 EGTA
-
 Leishmania amazonensis
1.14.13.39 Ngamma-nitro-L-arginine methyl ester
-
 Leishmania amazonensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+
-
 Leishmania amazonensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ Leishmania amazonensis
-
 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information Leishmania amazonensis the enzyme might be involved in the infectivity and/or escaping mechanism of the parasite ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Leishmania amazonensis
-
 constitutive enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 native enzyme from axenic amastigotes by 2',5'-ADP affinity chromatography to homogeneity Leishmania amazonensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 amastigote axenic, high enzyme expression level Leishmania amazonensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Leishmania amazonensis 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information the enzyme might be involved in the infectivity and/or escaping mechanism of the parasite Leishmania amazonensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.39 nitric oxide synthase
-
 Leishmania amazonensis
1.14.13.39 NOS
-
 Leishmania amazonensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 25
-
 assay at Leishmania amazonensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.4
-
 assay at Leishmania amazonensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 FAD
-
 Leishmania amazonensis
1.14.13.39 FMN
-
 Leishmania amazonensis
1.14.13.39 NADPH
-
 Leishmania amazonensis