EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.1.84 | environmental protection | NHase is used in two-step degradation (including amidase, EC 3.5.1.4) of acetonitrile-containing waste | Rhodococcus pyridinivorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.4 | 31.2 | - |
acetamide | - |
Rhodococcus sp. | |
3.5.1.4 | 39.2 | - |
acetic acid | - |
Rhodococcus sp. | |
3.5.1.4 | 1078 | - |
NH3 | - |
Rhodococcus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.4 | Rhodococcus sp. | - |
- |
- |
3.5.1.4 | Rhodococcus sp. S13-4 | - |
- |
- |
4.2.1.84 | Rhodococcus pyridinivorans | - |
strain: S82-2 | - |
4.2.1.84 | Rhodococcus pyridinivorans S82-2 | - |
strain: S82-2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.4 | - |
Rhodococcus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 187 | - |
- |
Rhodococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.4 | acetamide + H2O | amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process | Rhodococcus sp. | acetic acid + NH3 | - |
r | |
3.5.1.4 | acetamide + H2O | amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process | Rhodococcus sp. S13-4 | acetic acid + NH3 | - |
r | |
3.5.1.4 | acrylamide + H2O | 42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid | Rhodococcus sp. | acrylic acid + NH3 | - |
r | |
3.5.1.4 | acrylamide + H2O | 42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid | Rhodococcus sp. S13-4 | acrylic acid + NH3 | - |
r | |
3.5.1.4 | crotonic acid + NH3 | 5% of the activity with acetic acid. Reaction with crotonamide is 0.3% of the activity with acetamide | Rhodococcus sp. | crotonamide + H2O | - |
r | |
3.5.1.4 | formamide + H2O | 99% of the activity with acetamide | Rhodococcus sp. | formate + NH3 | - |
? | |
3.5.1.4 | formamide + H2O | 99% of the activity with acetamide | Rhodococcus sp. S13-4 | formate + NH3 | - |
? | |
3.5.1.4 | methacrylamide + H2O | 17% of the activity with acetamide. The activity of the reverse reaction is 0.1% of the activity with acetic acid | Rhodococcus sp. | methacrylic acid + NH3 | - |
? | |
3.5.1.4 | additional information | no activity with nicotinamide or benzamide | Rhodococcus sp. | ? | - |
? | |
3.5.1.4 | additional information | no activity with nicotinamide or benzamide | Rhodococcus sp. S13-4 | ? | - |
? | |
3.5.1.4 | n-butyramide + H2O | 75% of the activity with acetamide. The activity of the reverse reaction is 2% of the activity with acetic acid | Rhodococcus sp. | n-butyric acid + NH3 | - |
r | |
3.5.1.4 | propionamide + H2O | 79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid | Rhodococcus sp. | propionic acid + NH3 | - |
r | |
3.5.1.4 | propionamide + H2O | 79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid | Rhodococcus sp. S13-4 | propionic acid + NH3 | - |
r | |
4.2.1.84 | acetonitrile + H2O | - |
Rhodococcus pyridinivorans | acetamide | - |
r | |
4.2.1.84 | acetonitrile + H2O | - |
Rhodococcus pyridinivorans S82-2 | acetamide | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 30 | - |
acetamide hydrolysis | Rhodococcus sp. |
3.5.1.4 | 45 | - |
acetamide formation | Rhodococcus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.4 | 47.4 | - |
acetate | - |
Rhodococcus sp. | |
3.5.1.4 | 305 | - |
acetamide | - |
Rhodococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 7.5 | - |
acetamide hydrolysis | Rhodococcus sp. |
3.5.1.4 | 8 | - |
acetamide formation | Rhodococcus sp. |