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Literature summary extracted from

  • Kohyama, E.; Dohi, M.; Yoshimura, A.; Yoshida, T.; Nagasawa, T.
    Remaining acetamide in acetonitrile degradation using nitrile hydratase- and amidase-producing microorganisms (2007), Appl. Microbiol. Biotechnol., 74, 829-835.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.84 environmental protection NHase is used in two-step degradation (including amidase, EC 3.5.1.4) of acetonitrile-containing waste Rhodococcus pyridinivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 31.2
-
acetamide
-
Rhodococcus sp.
3.5.1.4 39.2
-
acetic acid
-
Rhodococcus sp.
3.5.1.4 1078
-
NH3
-
Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Rhodococcus sp.
-
-
-
3.5.1.4 Rhodococcus sp. S13-4
-
-
-
4.2.1.84 Rhodococcus pyridinivorans
-
strain: S82-2
-
4.2.1.84 Rhodococcus pyridinivorans S82-2
-
strain: S82-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4
-
Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 187
-
-
Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acetamide + H2O amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process Rhodococcus sp. acetic acid + NH3
-
r
3.5.1.4 acetamide + H2O amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process Rhodococcus sp. S13-4 acetic acid + NH3
-
r
3.5.1.4 acrylamide + H2O 42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid Rhodococcus sp. acrylic acid + NH3
-
r
3.5.1.4 acrylamide + H2O 42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid Rhodococcus sp. S13-4 acrylic acid + NH3
-
r
3.5.1.4 crotonic acid + NH3 5% of the activity with acetic acid. Reaction with crotonamide is 0.3% of the activity with acetamide Rhodococcus sp. crotonamide + H2O
-
r
3.5.1.4 formamide + H2O 99% of the activity with acetamide Rhodococcus sp. formate + NH3
-
?
3.5.1.4 formamide + H2O 99% of the activity with acetamide Rhodococcus sp. S13-4 formate + NH3
-
?
3.5.1.4 methacrylamide + H2O 17% of the activity with acetamide. The activity of the reverse reaction is 0.1% of the activity with acetic acid Rhodococcus sp. methacrylic acid + NH3
-
?
3.5.1.4 additional information no activity with nicotinamide or benzamide Rhodococcus sp. ?
-
?
3.5.1.4 additional information no activity with nicotinamide or benzamide Rhodococcus sp. S13-4 ?
-
?
3.5.1.4 n-butyramide + H2O 75% of the activity with acetamide. The activity of the reverse reaction is 2% of the activity with acetic acid Rhodococcus sp. n-butyric acid + NH3
-
r
3.5.1.4 propionamide + H2O 79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid Rhodococcus sp. propionic acid + NH3
-
r
3.5.1.4 propionamide + H2O 79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid Rhodococcus sp. S13-4 propionic acid + NH3
-
r
4.2.1.84 acetonitrile + H2O
-
Rhodococcus pyridinivorans acetamide
-
r
4.2.1.84 acetonitrile + H2O
-
Rhodococcus pyridinivorans S82-2 acetamide
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 30
-
acetamide hydrolysis Rhodococcus sp.
3.5.1.4 45
-
acetamide formation Rhodococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 47.4
-
acetate
-
Rhodococcus sp.
3.5.1.4 305
-
acetamide
-
Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 7.5
-
acetamide hydrolysis Rhodococcus sp.
3.5.1.4 8
-
acetamide formation Rhodococcus sp.