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Literature summary extracted from

  • Lin, L.L.; Chou, P.R.; Hua, Y.W.; Hsu, W.H.
    Overexpression, one-step purification, and biochemical characterization of a recombinant gamma-glutamyltranspeptidase from Bacillus licheniformis (2006), Appl. Microbiol. Biotechnol., 73, 103-112.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 expression of His6-tagged enzyme in Escherichia coli M15 Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 Ca2+ 1 mM, 22% inhibition Bacillus licheniformis
2.3.2.2 Hg2+ 1 mM 15% inhibition Bacillus licheniformis
2.3.2.2 Ni2+ 1 mM, 30% inhibition Bacillus licheniformis
2.3.2.2 Pb2+ 1 mM, 68% inhibition Bacillus licheniformis
2.3.2.2 Zn2+ 1 mM 24% inhibition Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.021
-
 5-L-glutamyl-4-nitroanilide
-
 Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.2 K+ 200 mM, 1.7fold increase in activity Bacillus licheniformis
2.3.2.2 Mg2+ 100 mM, 2fold increase in activity Bacillus licheniformis
2.3.2.2 Na+ 100 mM, 1.6fold increase in activity Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 20517
-
 x * 40475 + x * 20517, calculated from sequence Bacillus licheniformis
2.3.2.2 22000
-
 x * 41000 + x * 22000, SDS-PAGE Bacillus licheniformis
2.3.2.2 40475
-
 x * 40475 + x * 20517, calculated from sequence Bacillus licheniformis
2.3.2.2 41000
-
 x * 41000 + x * 22000, SDS-PAGE Bacillus licheniformis
2.3.2.2 60970
-
 calculated from sequence Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Bacillus licheniformis Q65KZ6 ATCC 27811
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 recombinant Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 5-L-glutamyl-4-nitroanilide + ethylamine
-
 Bacillus licheniformis N-ethyl-L-glutamine + 4-nitroaniline
-
 ?
2.3.2.2 D-Gln + ethylamine 1.3% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 gamma-Glu-L-Tyr + ethylamine 5.2% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 gamma-Glu-Leu + ethylamine 3.4% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 GSH + ethylamine 56.7% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 GSSG + ethylamine 10.6% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 L-Gln + ethylamine 18.8% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 L-Glu-gamma-3-carboxy-4-hydroxy-anilide + ethylamine 3.9% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 L-Glu-gamma-monohydroxamate + ethylamine 0.5% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 L-Glu-gamma-monomethyl ester + ethylamine 1.9% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 L-Glu-gamma-monomethyl ester + ethylamine 4.3% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?
2.3.2.2 S-methyl-glutathione + ethylamine 1.8% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 ? x * 40475 + x * 20517, calculated from sequence Bacillus licheniformis
2.3.2.2 ? x * 41000 + x * 22000, SDS-PAGE Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 40
-
-
 Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.2.2 30 50 30°C: about 85% of maximal activity, 50°C: about 80% of maximal activity Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.2 40
-
 30 min, pH 8.0, stable below Bacillus licheniformis
2.3.2.2 45
-
 30 min, pH 8.0, about 60% loss of activity Bacillus licheniformis
2.3.2.2 50
-
 30 min, complete inactivation Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.2 0.105
-
 5-L-glutamyl-4-nitroanilide
-
 Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 6 8
-
 Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.2 4 13 pH 4.0: about 50% of maximal activity, pH 13: about 40% of maximal activity Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.2.2 1 3 30 min, 4°C, about 75% loss of activity Bacillus licheniformis
2.3.2.2 4
-
 30 min, 4°C, about 80% loss of activity Bacillus licheniformis
2.3.2.2 5
-
 30 min, 4°C, about 20% loss of activity Bacillus licheniformis
2.3.2.2 6 8 30 min, 4°C, stable Bacillus licheniformis
2.3.2.2 9 12 30 min, 4°C, about 25% loss of activity Bacillus licheniformis