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Literature summary extracted from

  • Lindner, S.N.; Vidaurre, D.; Willbold, S.; Schoberth, S.M.; Wendisch, V.F.
    NCgl2620 encodes a class II polyphosphate kinase in Corynebacterium glutamicum (2007), Appl. Environ. Microbiol., 73, 5026-5033.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.1 (NH4)2SO4 optimal at 80 mM Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 genes NCgl0880 and NCgl2620 or pkk2A and pkk2B, expression of wild-type and mutant PKK2B isozymes in Escherichia coli strain Bl21(DE3), subcloning in Escherichia coli strain DH5alpha Corynebacterium glutamicum
2.7.4.1 overexpression in Escherichia coli Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 additional information ppk2A and ppk2b knockout organisms, ppk2b has PPK activity Corynebacterium glutamicum
2.7.4.1 additional information deletion of ppk2B decreases PPK activity and cellular polyphosphate content, while overexpression of ppk2B increases both PPK activity and cellular polyphosphate content. Neither deletion nor overexpression of ppk2A changes specific activity of PPK or cellular polyphosphate content significantly. The ppk2B deletion mutant, which accumulated very little polyphopshate and grows like the wild-type under phosphate-sufficient conditions, shows a growth defect under phosphate-limiting conditions, phenotype, overview Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 AMP
-
 Corynebacterium glutamicum
2.7.4.1 GMP
-
 Corynebacterium glutamicum
2.7.4.1 IMP
-
 Corynebacterium glutamicum
2.7.4.1 additional information no inhibition by UMP and CMP Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 0.14
-
 GTP
-
 Corynebacterium glutamicum
2.7.4.1 0.14
-
 GTP pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum
2.7.4.1 0.17
-
 ATP
-
 Corynebacterium glutamicum
2.7.4.1 0.17
-
 ATP pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum
2.7.4.1 1
-
 ITP
-
 Corynebacterium glutamicum
2.7.4.1 1
-
 ITP pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum
2.7.4.1 15
-
 (Phosphate)n
-
 Corynebacterium glutamicum
2.7.4.1 15
-
 Polyphosphate pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.1 cytosol
-
 Corynebacterium glutamicum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ optimal at 10 mM Corynebacterium glutamicum
2.7.4.1 Mg2+ optimal at 10 mM, activates Corynebacterium glutamicum
2.7.4.1 Mn2+ optimal at 10 mM Corynebacterium glutamicum
2.7.4.1 Mn2+ optimal at 10 mM, activates highly Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.1 151400
-
 gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ATP + (phosphate)n Corynebacterium glutamicum PPK2B forms polyphopshate with an average chain length of about 125 ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n Corynebacterium glutamicum MH20-22B PPK2B forms polyphopshate with an average chain length of about 125 ADP + (phosphate)n+1
-
 r
2.7.4.1 additional information Corynebacterium glutamicum accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview ?
-
 ?
2.7.4.1 additional information Corynebacterium glutamicum MH20-22B accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Corynebacterium glutamicum
-
 MH20-22B
-
2.7.4.1 Corynebacterium glutamicum
-
 wild-type strain ATCC 13032 is auxotrophic for biotin, genes NCgl0880 and NCgl2620 encoding class II isozymes PPK2A and PPK2B, no class I polyphosphate kinase, PPK1, encoded by ppk1, in this bacterium
-
2.7.4.1 Corynebacterium glutamicum MH20-22B
-
 MH20-22B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 of the overexpessed protein Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.1 additional information
-
-
 Corynebacterium glutamicum
2.7.4.1 14
-
 purified recombinant wild-type enzyme, polyphosphate synthesis with GTP Corynebacterium glutamicum
2.7.4.1 18
-
 purified recombinant wild-type enzyme, polyphosphate synthesis with ITP Corynebacterium glutamicum
2.7.4.1 31
-
 purified recombinant wild-type enzyme, polyphosphate synthesis with ATP Corynebacterium glutamicum
2.7.4.1 32
-
 purified recombinant wild-type enzyme, polyphosphate dephosphorylation Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n
-
 Corynebacterium glutamicum ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n PPK2B forms polyphopshate with an average chain length of about 125 Corynebacterium glutamicum ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n PPK2B forms polyphopshate with an average chain length of about 125 and prefers the polyphosphate synthesis reaction direction Corynebacterium glutamicum ADP + (phosphate)n+1 polyphosphate product chain length determination by NMR spectroscopy r
2.7.4.1 ATP + (phosphate)n
-
 Corynebacterium glutamicum MH20-22B ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n PPK2B forms polyphopshate with an average chain length of about 125 Corynebacterium glutamicum MH20-22B ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n PPK2B forms polyphopshate with an average chain length of about 125 and prefers the polyphosphate synthesis reaction direction Corynebacterium glutamicum MH20-22B ADP + (phosphate)n+1 polyphosphate product chain length determination by NMR spectroscopy r
2.7.4.1 ATP + ATP polyP not required as primer Corynebacterium glutamicum ADP + (phosphate)n+1
-
 r
2.7.4.1 GTP + (phosphate)n
-
 Corynebacterium glutamicum GDP + (phosphate)n+1
-
 r
2.7.4.1 GTP + (phosphate)n lower activity compared to ATP Corynebacterium glutamicum GDP + (phosphate)n+1
-
 r
2.7.4.1 ITP + (phosphate)n
-
 Corynebacterium glutamicum IDP + (phosphate)n+1
-
 r
2.7.4.1 ITP + (phosphate)n lower activity compared to ATP Corynebacterium glutamicum IDP + (phosphate)n+1
-
 r
2.7.4.1 additional information accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview Corynebacterium glutamicum ?
-
 ?
2.7.4.1 additional information PKK2B also shows NDP kinase activity Corynebacterium glutamicum ?
-
 ?
2.7.4.1 additional information accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview Corynebacterium glutamicum MH20-22B ?
-
 ?
2.7.4.1 additional information PKK2B also shows NDP kinase activity Corynebacterium glutamicum MH20-22B ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.1 homotetramer 4 x 39000, SDS PAGE Corynebacterium glutamicum
2.7.4.1 tetramer PPK2B is active as a homotetramer Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 ATP:polyphosphate phosphotransferase
-
 Corynebacterium glutamicum
2.7.4.1 class II polyphosphate kinase
-
 Corynebacterium glutamicum
2.7.4.1 kinase, polyphosphate (phosphorylating)
-
 Corynebacterium glutamicum
2.7.4.1 polyphosphate kinase
-
 Corynebacterium glutamicum
2.7.4.1 PPK
-
 Corynebacterium glutamicum
2.7.4.1 PPK2
-
 Corynebacterium glutamicum
2.7.4.1 PPK2B
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 30
-
 assay at Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.1 20
-
 preincubation results in a loss of 50% of the activity Corynebacterium glutamicum
2.7.4.1 20 25 30 min, purified recombinant wild-type PKK2B, 50% remaining activity Corynebacterium glutamicum
2.7.4.1 25
-
 preincubation results in a loss of 50% of the activity Corynebacterium glutamicum
2.7.4.1 45
-
 preincubation results in a loss of 50% of the activity with substrate Corynebacterium glutamicum
2.7.4.1 45
-
 preincubation results in an almost complete loss the activity without substrate Corynebacterium glutamicum
2.7.4.1 45
-
 30 min, purified recombinant wild-type PKK2B, almost no remaining activity in absence of polyphosphate, but about 50% remaining activity in presence of 40 mM polyphosphate Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.1 34
-
 GTP pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum
2.7.4.1 43
-
 ITP pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum
2.7.4.1 74
-
 ATP pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum
2.7.4.1 77
-
 Polyphosphate pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 7.2
-
 narrow optimum Corynebacterium glutamicum
2.7.4.1 7.2
-
 in 50mM TEA Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.1 ATP
-
 Corynebacterium glutamicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.1 3.9
-
 GMP
-
 Corynebacterium glutamicum
2.7.4.1 3.9
-
 GMP pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum
2.7.4.1 6.6
-
 IMP
-
 Corynebacterium glutamicum
2.7.4.1 6.6
-
 IMP pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum
2.7.4.1 7.9
-
 AMP no inhibition by CMP and UMP Corynebacterium glutamicum
2.7.4.1 7.9
-
 AMP pH 7.2, 30°C, recombinant wild-type enzyme Corynebacterium glutamicum