EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.164 | gene Fogal1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha as maltose-binding-protein fusion enzyme | Fusarium oxysporum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.164 | Ag+ | 1 mM, 50% inhibition | Fusarium oxysporum | |
3.2.1.164 | Cd2+ | 1 mM, 45% inhibition | Fusarium oxysporum | |
3.2.1.164 | Cu2+ | 1 mM, 45% inhibition | Fusarium oxysporum | |
3.2.1.164 | Fe2+ | 1 mM, 65% inhibition | Fusarium oxysporum | |
3.2.1.164 | Hg2+ | 1 mM, 80% inhibition | Fusarium oxysporum | |
3.2.1.164 | additional information | no inhibition by EDTA | Fusarium oxysporum | |
3.2.1.164 | Zn2+ | 1 mM, 60% inhibition | Fusarium oxysporum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.164 | additional information | - |
additional information | - |
Fusarium oxysporum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.164 | additional information | the enzyme activity does not require metal ions and is not affected by the presence of Ba2+, Ca2+, Co2+, Fe3+, K+, Mg2+, Mn2+, Na+, or Ni2+, overview | Fusarium oxysporum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.164 | 47000 | - |
x * 47000, SDS-PAGE | Fusarium oxysporum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.164 | Fusarium oxysporum | - |
gene Fogal1 | - |
3.2.1.164 | Fusarium oxysporum 12S | - |
gene Fogal1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.164 | recombinant maltose-binding-protein fusion enzyme from Escherichia coli strain DH5alpha, by cation exchange and amylose affinity chromatography, followed by hydrophobic interaction chromatography, native enzyme 88fold from strain S12 culture filtrate by anion exchange and hydrophobic interaction chromatography, followed by cation exchange chromatography, dialysis and gel filtration | Fusarium oxysporum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.164 | 1.55 | - |
purified native enzyme | Fusarium oxysporum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.164 | type II arabinogalactans + H2O | the enzyme preferentially debranches beta-1,6-galactobiose from the substrate, type II arabinogalactans from Western larch | Fusarium oxysporum | ? | - |
? | |
3.2.1.164 | type II arabinogalactans + H2O | the enzyme preferentially debranches beta-1,6-galactobiose from the substrate, type II arabinogalactans from Western larch | Fusarium oxysporum 12S | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.164 | ? | x * 47000, SDS-PAGE | Fusarium oxysporum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.164 | beta-1,6-galactanase | - |
Fusarium oxysporum |
3.2.1.164 | GAL1 | - |
Fusarium oxysporum |
3.2.1.164 | More | the enzyme belongs to the glycoside hydrolase family 5 | Fusarium oxysporum |
3.2.1.164 | type II arabinogalactan-degrading enzyme | - |
Fusarium oxysporum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.164 | 50 | - |
- |
Fusarium oxysporum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.164 | 60 | - |
1 h, purified native enzyme, over 80% remaining activity | Fusarium oxysporum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.164 | 5 | - |
- |
Fusarium oxysporum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.164 | 4 | 10 | 16 h, purified native enzyme, over 80% remaining activity | Fusarium oxysporum |