Literature summary extracted from

Tanous, C.; Chambellon, E.; Le Bars, D.; Delespaul, G.; Yvon, M.
Glutamate dehydrogenase activity can be transmitted naturally to Lactococcus lactis strains to stimulate amino acid conversion to aroma compounds (2006), Appl. Environ. Microbiol., 72, 1402-1409.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.4.1.4	additional information	exhibits 2.5-fold-higher GDH activity than cells are grown in milk, instead of glucose	Lactococcus lactis

Application

EC Number	Application	Comment	Organism
1.4.1.4	food industry	is responsible for the capacity of Lactococcus lactis strains to catabolize amino acids to aroma compounds, pGdh442 can be naturally transmitted to other Lactococcus lactis strains by using cadmium or zinc resistance as a selectable marker, new prospects to intensify and diversify the production of aroma compounds in cheese	Lactococcus lactis

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.4.1.4	overexpression with the NICE system in Lactococcus lactis TIL46 induced with increasing concentrations of nisin, pGdh442 transferred by electroporation (TIL504) or by conjugation (TIL507) in Lactococcus lactis MG1363 or in its derivative TIL193 strain containing pGhost8, appears to be stable after several subcultures of the strains without selection pressure, in the presence of plasmid pLP712, pGdh442 is unstable	Lactococcus lactis

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.4.1.4	additional information	gdh mutant TIL487, loss of ability to degrade amino acids	Lactococcus lactis

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.4.1.4	Lactococcus lactis	-	-	-
1.4.1.4	Lactococcus lactis NCDO1867	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.4.1.4	2-oxoglutarate + NADPH + NH3	-	Lactococcus lactis	L-glutamate + NADP+ + H2O	-	?
1.4.1.4	2-oxoglutarate + NADPH + NH3	-	Lactococcus lactis NCDO1867	L-glutamate + NADP+ + H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.4.1.4	GDH	-	Lactococcus lactis
1.4.1.4	glutamate dehydrogenase	-	Lactococcus lactis

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.4.1.4	NADPH	-	Lactococcus lactis

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Release 2023.1 (January 2023)