EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.1.50 | biotechnology | production of jojoba plant wax esters | Simmondsia chinensis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.50 | expression in Escherichia coli BL21(DE3) | Simmondsia chinensis |
2.3.1.75 | functional expression in Escherichia coli strain BL21(DE3) | Acinetobacter baylyi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.75 | additional information | establishment of heterologous wax ester biosynthesis in a recombinant Escherichia coli strain by coexpression of a fatty alcohol-producing bifunctional acyl-coenzyme A reductase from the jojoba plant, Simmondsia chinensis, and a bacterial wax ester synthase from Acinetobacter baylyi strain ADP1, catalyzing the esterification of fatty alcohols and coenzyme A thioesters of fatty acids, succesful production of jojoba oil-like wax esters, such as palmityl oleate, palmityl palmitoleate, and oleyl oleate, in presence of oleate, but also of fatty acid butyl esters, overview | Acinetobacter baylyi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.50 | Simmondsia chinensis | - |
- |
- |
2.3.1.75 | Acinetobacter baylyi | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.50 | palmitoyl-ACP + NADPH | the recombinant acyl-CoA reductase from Simmondsia chinensis exhibits a specificty to palmitoyl-ACP and not palmitoyl-CoA when expressed in Escherichia coli | Simmondsia chinensis | 1-hexadecanol + acyl-carrier protein + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.50 | acyl-CoA reductase | - |
Simmondsia chinensis |
2.3.1.75 | wax ester synthase | - |
Acinetobacter baylyi |