Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yanase, M.; Takata, H.; Fujii, K.; Takaha, T.; Kuriki, T.
    Cumulative effect of amino acid replacements results in enhanced thermostability of potato type L alpha-glucan phosphorylase (2005), Appl. Environ. Microbiol., 71, 5433-5439.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.1 F39L mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
2.4.1.1 F39L/N135S mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
2.4.1.1 F39L/N135S/T706I mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
2.4.1.1 F39L/T706I mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
2.4.1.1 N135S mutant with enhanced thermostability Solanum tuberosum
2.4.1.1 N135S/T706I mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
2.4.1.1 T706I mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 0.33
-
D-glucose-1-phosphate mutant T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.48
-
D-glucose-1-phosphate mutant F39L/N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.56
-
D-glucose-1-phosphate mutant N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.57
-
D-glucose-1-phosphate wild-type, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.59
-
D-glucose-1-phosphate mutant N135S, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.62
-
D-glucose-1-phosphate mutant F39L/N135S, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.76
-
D-glucose-1-phosphate mutant F39L/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.96
-
D-glucose-1-phosphate mutant F39L, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 1.54
-
maltotetraose mutant F39L/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 1.87
-
maltotetraose mutant T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 1.96
-
maltotetraose mutant N135S, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 2
-
maltotetraose mutant F39L, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 2
-
maltotetraose mutant F39L/N135S, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 2.11
-
maltotetraose wild-type, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 2.47
-
maltotetraose mutant N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 2.57
-
maltotetraose mutant F39L/N135S/T706I, 37°C, pH 5.5 Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 105000
-
2 * 105000, SDS-PAGE Solanum tuberosum
2.4.1.1 210000
-
PAGE Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Solanum tuberosum
-
type L alpha-glucan phosphorylase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 maltotetraose + D-glucose-1-phosphate
-
Solanum tuberosum maltopentaose + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 105000, SDS-PAGE Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 60
-
wild-type, complete loss of activity within 20 min. Mutants F39L, N135S and T706I retain about 50% of activity after 20 min at 60°C. Mutant F39L/N135S/T706I retains more than 95% of its activity after 60 min at 60°C Solanum tuberosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.1 0.001
-
D-glucose-1-phosphate wild-type, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0017
-
maltotetraose mutant F39L, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0019
-
D-glucose-1-phosphate mutant N135S, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.002
-
maltotetraose mutant N135S, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0023
-
D-glucose-1-phosphate mutant F39L, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0026
-
D-glucose-1-phosphate mutant T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0027
-
maltotetraose mutant F39L/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0029
-
maltotetraose mutant T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0036
-
maltotetraose mutant F39L/N135S, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0036
-
D-glucose-1-phosphate mutant F39L/N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0037
-
maltotetraose mutant F39L/N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0037
-
maltotetraose wild-type, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0041
-
D-glucose-1-phosphate mutant N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0042
-
D-glucose-1-phosphate mutant F39L/N135S, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0044
-
maltotetraose mutant N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
2.4.1.1 0.0049
-
D-glucose-1-phosphate mutant F39L/T706I, 37°C, pH 5.5 Solanum tuberosum