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Literature summary extracted from

  • Wiles, S.; Ferguson, K.; Stefanidou, M.; Young, D.B.; Robertson, B.D.
    Alternative luciferase for monitoring bacterial cells under adverse conditions (2005), Appl. Environ. Microbiol., 71, 3427-3432.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.3 gene Gluc, expression in Mycobacterium smegmatis using an hsp60 promoter, subcloning in Escherichia coli strain DH5alpha Gaussia princeps

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 coelenterazine + FMNH2 + O2 Gaussia princeps an imidazolopyrazine derivative CO2 + coelenteramide + FMN + light + H2O
-
 ir
1.14.14.3 additional information Gaussia princeps the Gluc luciferase retains its luminescence output in the stationary phase of growth and exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Gaussia princeps
-
 gene Gluc
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.14.14.3 the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature Gaussia princeps

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 coelenterazine + FMNH2 + O2 an imidazolopyrazine derivative Gaussia princeps CO2 + coelenteramide + FMN + light + H2O
-
 ir
1.14.14.3 additional information the Gluc luciferase retains its luminescence output in the stationary phase of growth and exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature Gaussia princeps ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.3 Gluc luciferase
-
 Gaussia princeps
1.14.14.3 luciferase
-
 Gaussia princeps

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.3 additional information
-
 the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature Gaussia princeps

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.14.3 additional information
-
 the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature Gaussia princeps

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.3 FMNH2
-
 Gaussia princeps