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Literature summary extracted from

  • Sapunova, L.I.; Lobanok, A.G.; Kazakevich, I.O.; Shlyakhotko, E.A.; Evtushenkov, A.N.
    Biosynthetic features and properties of xylose isomerases from Arthrobacter nicotianae, Escherichia coli, and Erwinia carotovora subsp. atroseptica (2006), Appl. Biochem. Microbiol., 42, 246-251.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.1.5 D-glucose if D-xylose is the carbon source D-glucose supplementation stimulates formation of xylose isomerase Glutamicibacter nicotianae
5.3.1.5 sucrose
-
 Glutamicibacter nicotianae
5.3.1.5 xylitol
-
 Escherichia coli
5.3.1.5 xylitol
-
 Glutamicibacter nicotianae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 D-glucose suppressed enzyme synthesis by increased content from 0.1 to 1.5%, productivity maxima at 0.1%, if D-xylose is the carbon source: D-glucose supplementation suppresses formation of xylose isomerase Escherichia coli
5.3.1.5 D-glucose suppressed enzyme synthesis by increased D-glucose content from 0.1 to 1.5%, productivity maxima at 1.0% Glutamicibacter nicotianae
5.3.1.5 D-glucose suppressed enzyme synthesis by increased D-glucose content from 0.1 to 1.5%, productivity maxima at 0.1% D-glucose, if D-xylose is the carbon source D-glucose supplementation suppresses formation of xylose isomerase Pectobacterium atrosepticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.5 D-Xylose Escherichia coli
-
 D-Xylulose
-
 r
5.3.1.5 D-Xylose Glutamicibacter nicotianae
-
 D-Xylulose
-
 r
5.3.1.5 D-Xylose Pectobacterium atrosepticum
-
 D-Xylulose
-
 r
5.3.1.5 D-Xylose Glutamicibacter nicotianae BIM B-5
-
 D-Xylulose
-
 r
5.3.1.5 D-Xylose Escherichia coli HB101xylA
-
 D-Xylulose
-
 r
5.3.1.5 D-Xylose Pectobacterium atrosepticum jn42xylA
-
 D-Xylulose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Escherichia coli
-
-
-
5.3.1.5 Escherichia coli HB101xylA
-
-
-
5.3.1.5 Glutamicibacter nicotianae
-
-
-
5.3.1.5 Glutamicibacter nicotianae BIM B-5
-
-
-
5.3.1.5 Pectobacterium atrosepticum
-
-
-
5.3.1.5 Pectobacterium atrosepticum jn42xylA
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5 PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Arthrobacter nicotianae and Erwinia carotovora subsp. atroseptica Escherichia coli
5.3.1.5 PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Escherichia coli and Erwinia carotovora subsp. atroseptica Glutamicibacter nicotianae
5.3.1.5 PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from from Arthrobacter nicotianae and Escherichia coli Pectobacterium atrosepticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 0.22
-
 enzyme production in the presence of D-glucose Escherichia coli
5.3.1.5 0.46
-
 crude extract Pectobacterium atrosepticum
5.3.1.5 0.46
-
 enzyme production in the presence of D-xylose Escherichia coli
5.3.1.5 0.47
-
 enzyme production in the presence of D-xylose Glutamicibacter nicotianae
5.3.1.5 0.71
-
 enzyme production in the presence of D-xylose Pectobacterium atrosepticum
5.3.1.5 0.73
-
 enzyme production in the presence of sucrose Glutamicibacter nicotianae
5.3.1.5 0.81
-
 enzyme production in the presence of D-glucose Pectobacterium atrosepticum
5.3.1.5 1.39
-
 enzyme production in the presence of xylitol Escherichia coli
5.3.1.5 1.63
-
 crude extract Glutamicibacter nicotianae
5.3.1.5 1.69
-
 crude extract Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-glucose lower efficiency Escherichia coli ?
-
 ?
5.3.1.5 D-glucose lower efficiency Glutamicibacter nicotianae ?
-
 ?
5.3.1.5 D-glucose lower efficiency Pectobacterium atrosepticum ?
-
 ?
5.3.1.5 D-glucose lower efficiency Glutamicibacter nicotianae BIM B-5 ?
-
 ?
5.3.1.5 D-glucose lower efficiency Escherichia coli HB101xylA ?
-
 ?
5.3.1.5 D-glucose lower efficiency Pectobacterium atrosepticum jn42xylA ?
-
 ?
5.3.1.5 D-Ribose weak effects Escherichia coli ?
-
 ?
5.3.1.5 D-Ribose weak effects Glutamicibacter nicotianae ?
-
 ?
5.3.1.5 D-Ribose weak effects Pectobacterium atrosepticum ?
-
 ?
5.3.1.5 D-Ribose weak effects Glutamicibacter nicotianae BIM B-5 ?
-
 ?
5.3.1.5 D-Ribose weak effects Escherichia coli HB101xylA ?
-
 ?
5.3.1.5 D-Ribose weak effects Pectobacterium atrosepticum jn42xylA ?
-
 ?
5.3.1.5 D-Xylose
-
 Escherichia coli D-Xylulose
-
 r
5.3.1.5 D-Xylose
-
 Glutamicibacter nicotianae D-Xylulose
-
 r
5.3.1.5 D-Xylose
-
 Pectobacterium atrosepticum D-Xylulose
-
 r
5.3.1.5 D-Xylose
-
 Glutamicibacter nicotianae BIM B-5 D-Xylulose
-
 r
5.3.1.5 D-Xylose
-
 Escherichia coli HB101xylA D-Xylulose
-
 r
5.3.1.5 D-Xylose
-
 Pectobacterium atrosepticum jn42xylA D-Xylulose
-
 r
5.3.1.5 L-Arabinose weak effects Escherichia coli ?
-
 ?
5.3.1.5 L-Arabinose weak effects Glutamicibacter nicotianae ?
-
 ?
5.3.1.5 L-Arabinose weak effects Pectobacterium atrosepticum ?
-
 ?
5.3.1.5 L-Arabinose weak effects Glutamicibacter nicotianae BIM B-5 ?
-
 ?
5.3.1.5 L-Arabinose weak effects Escherichia coli HB101xylA ?
-
 ?
5.3.1.5 L-Arabinose weak effects Pectobacterium atrosepticum jn42xylA ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.5 D-xylose: ketol-isomerase
-
 Escherichia coli
5.3.1.5 D-xylose: ketol-isomerase
-
 Glutamicibacter nicotianae
5.3.1.5 D-xylose: ketol-isomerase
-
 Pectobacterium atrosepticum
5.3.1.5 xylose isomerase
-
 Escherichia coli
5.3.1.5 xylose isomerase
-
 Glutamicibacter nicotianae
5.3.1.5 xylose isomerase
-
 Pectobacterium atrosepticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 70
-
-
 Escherichia coli
5.3.1.5 70
-
-
 Glutamicibacter nicotianae
5.3.1.5 75
-
-
 Pectobacterium atrosepticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 70
-
 pH 7.5, 2 h Escherichia coli
5.3.1.5 70
-
 pH 7.5, 2 h Glutamicibacter nicotianae
5.3.1.5 70 80 pH 7.8, 2 h, after following 2 h incubation at 80°C 37.6% loss of activity, 70-75% loss of activity at 80°C Pectobacterium atrosepticum
5.3.1.5 80
-
 2 h leads to 30.1% loss of activity Escherichia coli
5.3.1.5 80
-
 2 h leads to 30.5% loss of activity Glutamicibacter nicotianae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7.5
-
-
 Escherichia coli
5.3.1.5 7.5
-
-
 Glutamicibacter nicotianae
5.3.1.5 8
-
-
 Pectobacterium atrosepticum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.5 7 8 70°C for 1 h, 70-75% loss of activity at 80°C Escherichia coli
5.3.1.5 7 8 70°C for 1 h, 70-75% loss of activity at 80°C Glutamicibacter nicotianae
5.3.1.5 7 8 70°C for 1 h, 70-75% loss of activity at 80°C Pectobacterium atrosepticum