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Literature summary extracted from

  • Dias, M.V.; Faim, L.M.; Vasconcelos, I.B.; Simoes de Oliveira, J.; Basso, L.A.; Santos, D.S.; Filgueira de Azevedo, W.J.
    Effects of the magnesium and chloride ions and shikimate on the structure of shikimate kinase from Mycobacterium tuberculosis (2007), Acta Crystallogr. Sect. F, F63, 1-6.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.71
-
 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.71 hanging drop vapour diffusion method, enzyme in complex with ADP and shikimate in the absence of Mg2+ using 0.1 M Tris-HCl buffer pH 8.0, 17% PEG 1500 and 0.5-0.7 M LiCl, or enzyme in complex with ADP and Mg2+ in the absence of shikimate using 0.1 M Tris-HCl buffer pH 8.0, 20% PEG 3350 and 0.1 M MgCl2*6H2O Mycobacterium tuberculosis
2.7.1.71 in complex with ADP-shikimate and with MgADP-, at 1.93 A and 2.8 A resolution, respectively. Presence of Mg2+ influences the conformation of the shikimate hydroxyl groups and the position of the side chains of some of the residues of the activesite. Presence of Cl- seems to influence the affinity of ADP and its position in the active site and the opening length of the LID domain. Shikimate binding causes a closing of the LID domain and also seems to influence the crystallographic packing Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.71 Cl- increases the stability of the structure of the enzyme and influences the affinity of ADP for shikimate kinase Mycobacterium tuberculosis
2.7.1.71 Mg2+ influences the position of the hydroxyl groups of the shikimate molecule and some of the residues of the active site of the enzyme Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Mycobacterium tuberculosis P9WPY3
-
-
2.7.1.71 Mycobacterium tuberculosis H37Rv P9WPY3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.71
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 ATP + shikimate
-
 Mycobacterium tuberculosis ADP + shikimate 3-phosphate
-
 ?
2.7.1.71 ATP + shikimate
-
 Mycobacterium tuberculosis H37Rv ADP + shikimate 3-phosphate
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.71 ATP
-
 Mycobacterium tuberculosis