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Literature summary extracted from

  • Chen, C.D.; Huang, T.F.; Lin, C.H.; Guan, H.H.; Hsieh, Y.C.; Lin, Y.H.; Huang, Y.C.; Liu, M.Y.; Chang, W.C.; Chen, C.J.
    Purification, crystallization and preliminary X-ray crystallographic analysis of branched-chain aminotransferase from Deinococcus radiodurans (2007), Acta Crystallogr. Sect. F, 63, 492-494.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 expressed in Escherichia coli strain JM109 Deinococcus radiodurans
2.6.1.42 expression in Escherichia coli Deinococcus radiodurans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.42 diffraction to 2.5 A resolution, space group P212121 Deinococcus radiodurans
2.6.1.42 hanging drop vapour diffusion method using 20% (w/v) PEG 8000 and 0.2 M MgCl2 in 0.1 M Tris buffer pH 8.5 Deinococcus radiodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 40890
-
 ESI-tandem mass spectrometry Deinococcus radiodurans
2.6.1.42 40891
-
 x * 41000, SDS-PAGE, x * 40891, calculated Deinococcus radiodurans
2.6.1.42 40900
-
 SDS-PAGE Deinococcus radiodurans
2.6.1.42 41000
-
 x * 41000, SDS-PAGE, x * 40891, calculated Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Deinococcus radiodurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 His-tag Ni2+-NTA column chromatography Deinococcus radiodurans
2.6.1.42 recombinant protein Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Deinococcus radiodurans 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Deinococcus radiodurans 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-valine + 2-oxoglutarate
-
 Deinococcus radiodurans 3-methyl-2-oxobutanoate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.42 ? x * 41000, SDS-PAGE, x * 40891, calculated Deinococcus radiodurans

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BcaT
-
 Deinococcus radiodurans
2.6.1.42 branched-chain amino-acid aminotransferase
-
 Deinococcus radiodurans

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate required Deinococcus radiodurans