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Literature summary extracted from

  • Krengel, U.; Dey, R.; Sasso, S.; Okvist, M.; Ramakrishnan, C.; Kast, P.
    Preliminary X-ray crystallographic analysis of the secreted chorismate mutase from Mycobacterium tuberculosis: a tricky crystallization problem solved (2006), Acta Crystallogr. Sect. F, 62, 441-445.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 *MtCM prepared from Escherichia coli strain KA29 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.5 *MtCM, encoded by ORF Rv1885c in strain H37Rv. First characterized example of an AroQgamma fold. Description of the crystal optimization of a protein target that crystallizes very rapidly. 1. 175-residue version of *MtCM (encoded on plasmid pKTU3-HCT, dissolved in 20 mM potassium phosphate buffer pH7.5. 2. leaderless untagged 167-residue version of *MtCM encoded by plasmid pKTU3-HT used, buffered with 20 mM Tris-HCl pH 8.0) Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.5 37200
-
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.5 Chorismate Mycobacterium tuberculosis
-
 Prephenate
-
 ?
5.4.99.5 Chorismate Mycobacterium tuberculosis H37Rv
-
 Prephenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Mycobacterium tuberculosis P9WIB9 encoded by Rv1885c
-
5.4.99.5 Mycobacterium tuberculosis H37Rv P9WIB9 encoded by Rv1885c
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Mycobacterium tuberculosis Prephenate
-
 ?
5.4.99.5 Chorismate
-
 Mycobacterium tuberculosis H37Rv Prephenate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 dimer
-
 Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
5.4.99.5 chorismate mutase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.5 20
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.5 7.5
-
 assay at Mycobacterium tuberculosis