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Literature summary extracted from

  • Henriksson, L.M.; Bjoerkelid, C.; Mowbray, S.L.; Unge, T.
    The 1.9 A resolution structure of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a potential drug target (2006), Acta Crystallogr. Sect. D, D62, 807-813.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.267 drug development the enzyme is an excellent drug target in a number of pathogenic organisms Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 gene Rv2870c, expression of His-tagged enzyme in Escherichia coli strain BL21 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.267 purified recombinant His-tagged enzyme with inhibitor fosmidomycin, microbatch method, sitting drops mixed from 0.001 ml of protein solution, containing 5 mg/ml protein in 150 mM NaCl and 20 mM Tris–HCl pH 7.5, and 0.001 ml of crystallization buffer, containing 40% ethylene glycol, 2 mM MgSO4, 20 mM DTT, 0.2 mM EDTA and 100 mM acetate pH 5.0, streak-seeded immediately with a horse hair, 4 weeks, stabilization prior to flash-cooling in reservoir solution complemented with additional 10% ethylene glycol and 1 mM fosmidomycin, X-ray diffraction structure determination and analysis at 1.9 A resolution, molecular replacement Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 fosmidomycin
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.0072
-
 NADPH
-
 Mycobacterium tuberculosis
1.1.1.267 0.34
-
 1-deoxy-D-xylulose 5-phosphate
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mn2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Mycobacterium tuberculosis second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Mycobacterium tuberculosis H37Rv second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Mycobacterium tuberculosis
-
 gene Rv2870c
-
1.1.1.267 Mycobacterium tuberculosis H37Rv
-
 gene Rv2870c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.267 recombinant His-tagged enzyme from Escherichia coli strain BL21 by metal ion affinity chromatography, and gel filtration, to 98% purity Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.267 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ active site structure Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.267 dimer enzyme crystal structure, the dimer interface is created by interactions between the catalytic and connecting regions of each subunit, a sulfate is bound at the expected site of the phosphate moiety of the sugar substrate, molecular replacement structure analysis and comparison, overview Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 DXR
-
 Mycobacterium tuberculosis
1.1.1.267 Rv2870c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 22
-
 assay at Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.267 5.3
-
 NADPH
-
 Mycobacterium tuberculosis
1.1.1.267 5.3
-
 1-deoxy-D-xylulose 5-phosphate
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADPH
-
 Mycobacterium tuberculosis