Literature summary extracted from
Smith, C.A.; Cross, J.A.; Bognar, A.L.; Sun, X.
Mutation of Gly51 to serine in the P-loop of Lactobacillus casei folylpolyglutamate synthetase abolishes activity by altering the conformation of two adjacent loops (2006), Acta Crystallogr. Sect. D, 62, 548-558.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
6.3.2.17 |
expressed in Escherichia coli strain BL21 |
Lacticaseibacillus casei |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
6.3.2.17 |
hanging drop vapour diffusion method using 50 mM acetate buffer pH 5.3 and 6% PEG 4000 |
Lacticaseibacillus casei |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
6.3.2.17 |
G51S |
no FGPS activity |
Lacticaseibacillus casei |
6.3.2.17 |
G51S/S52T |
no FGPS activity |
Lacticaseibacillus casei |
6.3.2.17 |
S73A |
mutant retains some FGPS activity |
Lacticaseibacillus casei |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
6.3.2.17 |
Mg2+ |
essential |
Lacticaseibacillus casei |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.3.2.17 |
Lacticaseibacillus casei |
P15925 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
6.3.2.17 |
chitin column chromatography |
Lacticaseibacillus casei |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.3.2.17 |
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu |
- |
Lacticaseibacillus casei |
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2 |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
6.3.2.17 |
Folylpolyglutamate synthetase |
- |
Lacticaseibacillus casei |
6.3.2.17 |
FPGS |
- |
Lacticaseibacillus casei |