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Literature summary extracted from

  • Polz, L.; Schubel, H.; Stockigt, J.
    Characterization of 2beta(R)-17-O-acetylajmalan: acetylesterase - a specific enzyme involved in the biosynthesis of the Rauwolfia alkaloid ajmaline (1987), Z. Naturforsch. C, 42, 333-342.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.80 4°C, at the stage of a 150fold enrichment in 0.1 M phosphate buffer, pH 7.0, 1 mM dithiothreitol, half-life: 21 days Rauvolfia serpentina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.80 2,2'-dithiodipyridine 0.5 mM, 30-40% inhibition Rauvolfia serpentina
3.1.1.80 5,5'-dithiobis(2-nitrobenzoic acid) 0.5 mM, 30-40% inhibition Rauvolfia serpentina
3.1.1.80 iodoacetamide 0.5 mM, 30-40% inhibition Rauvolfia serpentina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.80 33000
-
 gel filtration Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.80 Rauvolfia serpentina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.80 partial Rauvolfia serpentina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.80 cell suspension culture
-
 Rauvolfia serpentina
-
3.1.1.80 leaf
-
 Rauvolfia serpentina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.80 0.0364
-
-
 Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.80 17-O-acetylajmaline + H2O 84.2% of the activity with 17-O-acetylnorajmaline Rauvolfia serpentina ajmaline + acetate
-
 ?
3.1.1.80 17-O-acetylnorajmaline + H2O
-
 Rauvolfia serpentina norajmaline + acetate
-
 ?
3.1.1.80 17-O-acetyltetraphyllicine + H2O 86% of the activity with 17-O-acetylnorajmaline Rauvolfia serpentina ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.80 37
-
-
 Rauvolfia serpentina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.80 7.5
-
-
 Rauvolfia serpentina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.80 6.3 8.5 pH 6.3: about 50% of maximal activity, pH 8.5: about 50% of maximal activity Rauvolfia serpentina

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.80 Rauvolfia serpentina isoelectric focusing
-
 4.9