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Literature summary extracted from

  • Yanee, T.; Suwanna, N.; Sineenat, S.
    Vitamin B6 degradation by pyridoxamine-pyruvate transaminase and pyridoxine 4-oxidase from Ochrobactrum anthropi- and Enterobacter cloacae-like bacteria (2005), Science Asia, 31, 307-311.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.12 pyridoxine + O2 Enterobacter cloacae the enzyme is involved in degradation of vitamin B6 via pathway I pyridoxal + H2O2
-
?
1.1.3.12 pyridoxine + O2 Brucella anthropi the enzyme is involved in degradation of vitamin B6 via pathway I pyridoxal + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.12 Brucella anthropi
-
isolate T2
-
1.1.3.12 Enterobacter cloacae
-
isolate T6
-
2.6.1.30 Brucella anthropi
-
Gram-negative bacillus isolate T2, 99.9% related to Ochrobactrum anthropi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.12 0.0000041
-
isolate T2 Brucella anthropi
1.1.3.12 0.0000094
-
isolate T6 Enterobacter cloacae
2.6.1.30 0.00631
-
at 30°C Brucella anthropi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.12 pyridoxine + O2
-
Enterobacter cloacae pyridoxal + H2O2
-
?
1.1.3.12 pyridoxine + O2
-
Brucella anthropi pyridoxal + H2O2
-
?
1.1.3.12 pyridoxine + O2 the enzyme is involved in degradation of vitamin B6 via pathway I Enterobacter cloacae pyridoxal + H2O2
-
?
1.1.3.12 pyridoxine + O2 the enzyme is involved in degradation of vitamin B6 via pathway I Brucella anthropi pyridoxal + H2O2
-
?
2.6.1.30 pyridoxamine + pyruvate
-
Brucella anthropi pyridoxal + L-alanine
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.12 PN 4-oxidase
-
Enterobacter cloacae
1.1.3.12 PN 4-oxidase
-
Brucella anthropi
2.6.1.30 PM-pyruvate transaminase
-
Brucella anthropi
2.6.1.30 pyridoxamine-pyruvate aminotransferase
-
Brucella anthropi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.12 30
-
assay at Enterobacter cloacae
1.1.3.12 30
-
assay at Brucella anthropi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.12 7
-
assay at Enterobacter cloacae
1.1.3.12 7
-
assay at Brucella anthropi

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.12 FAD required Enterobacter cloacae
1.1.3.12 FAD required Brucella anthropi
2.6.1.30 pyridoxal 5'-phosphate
-
Brucella anthropi