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Literature summary extracted from

  • Fonseca, I.O.; Magalhaes, M.L.; Oliveira, J.S.; Silva, R.G.; Mendes, M.A.; Palma, M.S.; Santos, D.S.; Basso, L.A.
    Functional shikimate dehydrogenase from Mycobacterium tuberculosis H37Rv: purification and characterization (2006), Protein Expr. Purif., 46, 429-437.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 gene aroE, DNA and amino acid sequence determination, functional expression as soluble enzyme in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.25 additional information
-
 additional information steady-state kinetics of recombinant AroE Mycobacterium tuberculosis
1.1.1.25 0.01
-
 NADPH pH 7.0, 25°C, recombinant AroE Mycobacterium tuberculosis
1.1.1.25 0.022
-
 NADP+ pH 7.0, 25°C, recombinant AroE Mycobacterium tuberculosis
1.1.1.25 0.031
-
 3-dehydroshikimate pH 7.0, 25°C, recombinant AroE Mycobacterium tuberculosis
1.1.1.25 0.05
-
 shikimate pH 7.0, 25°C, recombinant AroE Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.25 27000
-
 1 * 27000, recombinant AroE, SDS-PAGE, 1 * 27076, recombinant AroE, mass spectrometry Mycobacterium tuberculosis
1.1.1.25 27076
-
 1 * 27000, recombinant AroE, SDS-PAGE, 1 * 27076, recombinant AroE, mass spectrometry Mycobacterium tuberculosis
1.1.1.25 27080
-
 recombinant AroE, mass spectrometry Mycobacterium tuberculosis
1.1.1.25 54150
-
 recombinant AroE, mass spectrometry Mycobacterium tuberculosis
1.1.1.25 58370
-
 recombinant AroE, gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Mycobacterium tuberculosis A5U5Q2 gene aroE
-
1.1.1.25 Mycobacterium tuberculosis H37Rv A5U5Q2 gene aroE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.25 recombinant soluble enzyme 8.5fold from Escherichia coli strain BL21(DE3) by ion exchange and hydrophobic interaction chromatography, gel filtration, and again ion exchange chromatography, to homogeneity Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.25 3.9
-
 purified recombinant AroE Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 shikimate + NADP+
-
 Mycobacterium tuberculosis 3-dehydroshikimate + NADPH + H+
-
 ?
1.1.1.25 shikimate + NADP+
-
 Mycobacterium tuberculosis H37Rv 3-dehydroshikimate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.25 dimer 2 * 54150, recombinant AroE, mass spectrometry Mycobacterium tuberculosis
1.1.1.25 monomer 1 * 27000, recombinant AroE, SDS-PAGE, 1 * 27076, recombinant AroE, mass spectrometry Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.25 AroE
-
 Mycobacterium tuberculosis
1.1.1.25 SD
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.25 25
-
 assay at Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.25 37
-
 purified recombinant AroE, stable for at least 1 h Mycobacterium tuberculosis
1.1.1.25 55
-
 purified recombinant AroE, 20% remaining activity after 1 h Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.25 5.9
-
 NADP+ pH 7.0, 25°C, recombinant AroE Mycobacterium tuberculosis
1.1.1.25 8.2
-
 shikimate pH 7.0, 25°C, recombinant AroE Mycobacterium tuberculosis
1.1.1.25 45
-
 NADPH pH 7.0, 25°C, recombinant AroE Mycobacterium tuberculosis
1.1.1.25 49
-
 3-dehydroshikimate pH 7.0, 25°C, recombinant AroE Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 7
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NADP+
-
 Mycobacterium tuberculosis