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Literature summary extracted from

  • El Hassouni, M.; Chambost, J.P.; Expert, D.; Van Gijsegem, F.; Barras, F.
    The minimal gene set member msrA, encoding peptide methionine sulfoxide reductase, is a virulence determinant of the plant pathogen Erwinia chrysanthemi (1999), Proc. Natl. Acad. Sci. USA, 96, 887-892.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 gene msrA, DNA and amino acid sequence determination and analysis, expression of His6-tagged MsrA in Escherichia coli strain BL21 Dickeya chrysanthemi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.11 additional information Dickeya chrysanthemi MsrA is a virulence determinant for the plant pathogen required for full virulence ?
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin Dickeya chrysanthemi stereospecific reduction of protein-bound methionine (S)-sulfoxide residues, the enzyme is involved in oxidized protein repair peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Dickeya chrysanthemi
-
 gene msrA, wild-type strain 3937 or RH7006 or pin14
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.11 recombinant His6-tagged MsrA from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity Dickeya chrysanthemi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.11 additional information
-
 virulence on chicory leaves of wild-type and recombinant strains, overview Dickeya chrysanthemi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 additional information MsrA is a virulence determinant for the plant pathogen required for full virulence Dickeya chrysanthemi ?
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin stereospecific reduction of protein-bound methionine (S)-sulfoxide residues, the enzyme is involved in oxidized protein repair Dickeya chrysanthemi peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin stereospecific reduction of protein-bound methionine (S)-sulfoxide residues Dickeya chrysanthemi peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 MsrA
-
 Dickeya chrysanthemi
1.8.4.11 peptide methionine sulfoxide reductase
-
 Dickeya chrysanthemi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.11 25
-
 assay at Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.11 7.4
-
 assay at Dickeya chrysanthemi

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 thioredoxin
-
 Dickeya chrysanthemi