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Literature summary extracted from
- L-tartaric acid synthesis from vitamin C in higher plants (2006), Proc. Natl. Acad. Sci. USA, 103, 5608-5613.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.264 | gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Vitis vinifera |
| 1.1.1.264 | gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Vitis californica |
| 1.1.1.264 | gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Ampelopsis glandulosa var. brevipedunculata |
| 1.1.1.366 | expressed in Escherichia coli BL21(DE3) pLysS cells | Vitis vinifera |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.264 | 2.2 | - |
L-Idonate | pH 5.4, 30°C, recombinant enzyme | Vitis vinifera |  |
| 1.1.1.264 | 12.5 | - |
5-Dehydro-D-gluconate | pH 8.5, 30°C, recombinant enzyme | Vitis vinifera | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.366 | 40000 | - |
x * 40000, SDS-PAGE | Vitis vinifera |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.264 | L-idonate + NADP+ | Vitis vinifera | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | 5-dehydro-D-gluconate + NADPH | - |
r | |
| 1.1.1.264 | L-idonate + NADP+ | Vitis californica | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | 5-dehydro-D-gluconate + NADPH | - |
r | |
| 1.1.1.264 | L-idonate + NADP+ | Ampelopsis glandulosa var. brevipedunculata | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | 5-dehydro-D-gluconate + NADPH | - |
r | |
| 1.1.1.366 | L-idonate + NAD+ | Vitis vinifera | - |
5-dehydro-D-gluconate + NADH + H+ | - |
r | |
| 1.1.1.366 | additional information | Vitis vinifera | no activity with NADPH and NADP+ | ? | - |
? | |
| 1.1.1.366 | sorbitol + NAD+ | Vitis vinifera | when sorbitol is tested as a substrate, NAD+ reduction occurs but at a very low rate | ? | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.264 | Ampelopsis glandulosa var. brevipedunculata | - |
- |
- |
| 1.1.1.264 | no activity in Ampelopsis aconitifolia | - |
- |
- |
| 1.1.1.264 | Vitis californica | - |
- |
- |
| 1.1.1.264 | Vitis vinifera | Q1PSI9 | cv. Cabernet Sauvignon | - |
| 1.1.1.366 | Vitis vinifera | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.264 | recombinant enzyme from Escherichia coli strain BL21(DE3) | Vitis vinifera |
| 1.1.1.264 | recombinant enzyme from Escherichia coli strain BL21(DE3) | Vitis californica |
| 1.1.1.264 | recombinant enzyme from Escherichia coli strain BL21(DE3) | Ampelopsis glandulosa var. brevipedunculata |
| 1.1.1.366 | - |
Vitis vinifera |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.264 | fruit | - |
Vitis vinifera | - |
| 1.1.1.264 | fruit | - |
Vitis californica | - |
| 1.1.1.264 | fruit | - |
Ampelopsis glandulosa var. brevipedunculata | - |
| 1.1.1.264 | leaf | young | Vitis vinifera | - |
| 1.1.1.264 | leaf | young | Vitis californica | - |
| 1.1.1.264 | leaf | young | Ampelopsis glandulosa var. brevipedunculata | - |
| 1.1.1.366 | fruit | - |
Vitis vinifera | - |
| 1.1.1.366 | leaf | - |
Vitis vinifera | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.264 | additional information | - |
in vivo activity, feeding experiments, overview | Vitis vinifera |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.264 | L-idonate + NADP+ | - |
Vitis vinifera | 5-dehydro-D-gluconate + NADPH | i.e. 5-keto-D-gluconic acid | r | |
| 1.1.1.264 | L-idonate + NADP+ | - |
Vitis californica | 5-dehydro-D-gluconate + NADPH | i.e. 5-keto-D-gluconic acid | r | |
| 1.1.1.264 | L-idonate + NADP+ | - |
Ampelopsis glandulosa var. brevipedunculata | 5-dehydro-D-gluconate + NADPH | i.e. 5-keto-D-gluconic acid | r | |
| 1.1.1.264 | L-idonate + NADP+ | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | Vitis vinifera | 5-dehydro-D-gluconate + NADPH | - |
r | |
| 1.1.1.264 | L-idonate + NADP+ | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | Vitis californica | 5-dehydro-D-gluconate + NADPH | - |
r | |
| 1.1.1.264 | L-idonate + NADP+ | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | Ampelopsis glandulosa var. brevipedunculata | 5-dehydro-D-gluconate + NADPH | - |
r | |
| 1.1.1.366 | L-idonate + NAD+ | - |
Vitis vinifera | 5-dehydro-D-gluconate + NADH + H+ | - |
r | |
| 1.1.1.366 | additional information | no activity with NADPH and NADP+ | Vitis vinifera | ? | - |
? | |
| 1.1.1.366 | sorbitol + NAD+ | when sorbitol is tested as a substrate, NAD+ reduction occurs but at a very low rate | Vitis vinifera | ? | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.366 | ? | x * 40000, SDS-PAGE | Vitis vinifera |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.264 | L-IdnDH | - |
Vitis vinifera |
| 1.1.1.264 | L-IdnDH | - |
Vitis californica |
| 1.1.1.264 | L-IdnDH | - |
Ampelopsis glandulosa var. brevipedunculata |
| 1.1.1.264 | L-idonate dehydrogenase | - |
Vitis vinifera |
| 1.1.1.264 | L-idonate dehydrogenase | - |
Vitis californica |
| 1.1.1.264 | L-idonate dehydrogenase | - |
Ampelopsis glandulosa var. brevipedunculata |
| 1.1.1.366 | L-IdnDH | - |
Vitis vinifera |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.264 | 30 | - |
assay at | Vitis vinifera |
| 1.1.1.264 | 30 | - |
assay at | Vitis californica |
| 1.1.1.264 | 30 | - |
assay at | Ampelopsis glandulosa var. brevipedunculata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.264 | 5.4 | - |
forward, oxidation reaction | Vitis vinifera |
| 1.1.1.264 | 5.4 | - |
forward, oxidation reaction | Vitis californica |
| 1.1.1.264 | 5.4 | - |
forward, oxidation reaction | Ampelopsis glandulosa var. brevipedunculata |
| 1.1.1.264 | 8.5 | - |
reverse, reduction reaction | Vitis vinifera |
| 1.1.1.264 | 8.5 | - |
reverse, reduction reaction | Vitis californica |
| 1.1.1.264 | 8.5 | - |
reverse, reduction reaction | Ampelopsis glandulosa var. brevipedunculata |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.264 | NADP+ | - |
Vitis vinifera | |
| 1.1.1.264 | NADP+ | - |
Vitis californica | |
| 1.1.1.264 | NADP+ | - |
Ampelopsis glandulosa var. brevipedunculata | |
| 1.1.1.264 | NADPH | - |
Vitis vinifera | |
| 1.1.1.264 | NADPH | - |
Vitis californica | |
| 1.1.1.264 | NADPH | - |
Ampelopsis glandulosa var. brevipedunculata | |
| 1.1.1.366 | NAD+ | - |
Vitis vinifera | |
| 1.1.1.366 | NADH | - |
Vitis vinifera | |
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