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Literature summary extracted from
- Expression profile analysis and biochemical properties of the peptide methionine sulfoxide reductase A (PMSRA) gene family in Arabidopsis (2006), Plant Sci., 170, 705-714.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | additional information | isozyme PMSRA2 is slightly induced by paraquat, ozone, and high light conditions | Arabidopsis thaliana | |
| 1.8.4.11 | additional information | isozyme PMSRA3 expression is slightly induced by ozone | Arabidopsis thaliana | |
| 1.8.4.11 | additional information | isozyme PMSRA4 is highly induced by high light conditions, and slightly by ozone, paraquat, and cercosporin | Arabidopsis thaliana |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | locus At2g18030, isozyme PMSRA5, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21(DE3), prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
| 1.8.4.11 | locus At4g25130, isozyme PMSRA4, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged isozyme PMSRA4 in Escherichia coli, prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
| 1.8.4.11 | locus At5g07460, isozyme PMSRA2, DNA and amino acid sequence determination and analysis, phylogenetic tree, prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
| 1.8.4.11 | locus At5g07470, isozyme PMSRA3, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged isozyme PMSRA3 in Escherichia coli, prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
| 1.8.4.11 | locus At5g61640, isozyme PMSRA1, DNA and amino acid sequence determination and analysis, phylogenetic tree, prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | additional information | isozyme PMSRA5 expression is slightly suppressed by ozone | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.11 | 1.1 | - |
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide | recombinant isozyme PMSRA4, with DTT, pH 8.0, 22°C | Arabidopsis thaliana |  |
| 1.8.4.11 | 2.12 | - |
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide | recombinant isozyme PMSRA3, with DTT, pH 7.5, 22°C | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.4.11 | chloroplast | isozyme PMSRA3 | Arabidopsis thaliana | 9507 | - |
| 1.8.4.11 | chloroplast | isozyme PMSRA4 | Arabidopsis thaliana | 9507 | - |
| 1.8.4.11 | cytosol | cytosolic isozyme PMsrA1 | Arabidopsis thaliana | 5829 | - |
| 1.8.4.11 | cytosol | isozyme PMSRA2 | Arabidopsis thaliana | 5829 | - |
| 1.8.4.11 | additional information | subcellular localization of isozymes, overview | Arabidopsis thaliana | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | peptide-L-methionine-(S)-S-oxide + thioredoxin | Arabidopsis thaliana | stereospecific reduction | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Arabidopsis thaliana | P54150 | isozyme PMSRA1 | - |
| 1.8.4.11 | Arabidopsis thaliana | P54150 | isozyme PMSRA2 | - |
| 1.8.4.11 | Arabidopsis thaliana | P54150 | isozyme PMSRA3 | - |
| 1.8.4.11 | Arabidopsis thaliana | P54150 | isozyme PMSRA4 | - |
| 1.8.4.11 | Arabidopsis thaliana | Q9SL43 | isozyme PMSRA5 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | recombinant His-tagged isozyme PMSRA3 from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
| 1.8.4.11 | recombinant His-tagged isozyme PMSRA4 from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | cauline leaf | - |
Arabidopsis thaliana | - |
| 1.8.4.11 | cauline leaf | highest expression level of isozyme PMSRA4 | Arabidopsis thaliana | - |
| 1.8.4.11 | flower | mature, after anthesis | Arabidopsis thaliana | - |
| 1.8.4.11 | flower bud | - |
Arabidopsis thaliana | - |
| 1.8.4.11 | leaf | young and mature | Arabidopsis thaliana | - |
| 1.8.4.11 | additional information | quantitative expression profile analysis | Arabidopsis thaliana | - |
| 1.8.4.11 | root | - |
Arabidopsis thaliana | - |
| 1.8.4.11 | root | highest expression level of isozyme PMSRA3 | Arabidopsis thaliana | - |
| 1.8.4.11 | seedling | germinated | Arabidopsis thaliana | - |
| 1.8.4.11 | seedling | germinated, highest expression level of isozyme PMSRA4 | Arabidopsis thaliana | - |
| 1.8.4.11 | stem | - |
Arabidopsis thaliana | - |
| 1.8.4.11 | stem | highest expression level of isozyme PMSRA2 | Arabidopsis thaliana | - |
| 1.8.4.11 | stem | highest expression level of isozyme PMSRA5 | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | Fmoc-L-methionine (S)-sulfoxide + dithiothreitol | - |
Arabidopsis thaliana | Fmoc-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine-(S)-S-oxide + DTT | stereospecific reduction, 9-fluorenylmethyl chloroformate-labeled substrate | Arabidopsis thaliana | L-methionine + DTT disulfide + H2O | - |
? | |
| 1.8.4.11 | peptide-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction | Arabidopsis thaliana | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | More | the enzyme belongs to the peptide methionine sulfoxide reductase A, PMSRA, gene family | Arabidopsis thaliana |
| 1.8.4.11 | peptide methionine sulfoxide reductase A | - |
Arabidopsis thaliana |
| 1.8.4.11 | PMSRA | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 22 | - |
assay at | Arabidopsis thaliana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.11 | 0.91 | - |
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide | recombinant isozyme PMSRA3, with DTT, pH 7.5, 22°C | Arabidopsis thaliana | |
| 1.8.4.11 | 1.59 | - |
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide | recombinant isozyme PMSRA4, with DTT, pH 8.0, 22°C | Arabidopsis thaliana | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 7.5 | - |
assay at | Arabidopsis thaliana |
| 1.8.4.11 | 7.5 | 8 | assay at | Arabidopsis thaliana |
| 1.8.4.11 | 8 | - |
assay at | Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | dithiothreitol | - |
Arabidopsis thaliana | |
| 1.8.4.11 | thioredoxin | - |
Arabidopsis thaliana | |
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