EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.207 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Mentha x piperita |
1.1.1.208 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Mentha x piperita |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.207 | 0.00012 | - |
NADPH | pH 7.0, 31°C, recombinant enzyme | Mentha x piperita | |
1.1.1.207 | 0.003 | - |
(-)-menthone | pH 7.0, 31°C, recombinant enzyme | Mentha x piperita | |
1.1.1.207 | 0.041 | - |
(+)-isomenthone | pH 7.0, 31°C, recombinant enzyme | Mentha x piperita | |
1.1.1.208 | 0.0054 | - |
NADP+ | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita | |
1.1.1.208 | 0.01 | - |
NADPH | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita | |
1.1.1.208 | 0.674 | - |
(-)-menthone | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita | |
1.1.1.208 | 1 | - |
(+)-isomenthone | above, pH 9.3, 31°C, recombinant enzyme | Mentha x piperita |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.207 | 34070 | - |
x * 34070, amino acid sequence calculation | Mentha x piperita |
1.1.1.208 | 35722 | - |
x * 35722, amino acid sequence calculation | Mentha x piperita |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.207 | (+)-isomenthone + NADPH | Mentha x piperita | - |
(+)-neoisomenthol + NADP+ | - |
? | |
1.1.1.207 | (-)-menthone + NADPH | Mentha x piperita | - |
(3R)-menthol + NADP+ | - |
? | |
1.1.1.207 | additional information | Mentha x piperita | primary monoterpene biosynthesis pathway, overview | ? | - |
? | |
1.1.1.208 | (+)-isomenthone + NADPH | Mentha x piperita | low activity | (+)-isomenthol + NADP+ | - |
? | |
1.1.1.208 | (-)-menthone + NADPH + H+ | Mentha x piperita | - |
(3S)-neomenthol + NADP+ | - |
r | |
1.1.1.208 | additional information | Mentha x piperita | primary monoterpene biosynthesis pathway, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.207 | Mentha x piperita | - |
cv. Black Mitchum | - |
1.1.1.208 | Mentha x piperita | - |
cv. Black Mitchum | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.207 | recombinant enzyme partially | Mentha x piperita |
1.1.1.208 | recombinant enzyme partially | Mentha x piperita |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.207 | leaf | - |
Mentha x piperita | - |
1.1.1.207 | oil gland secretory cell | - |
Mentha x piperita | - |
1.1.1.208 | leaf | - |
Mentha x piperita | - |
1.1.1.208 | oil gland secretory cell | - |
Mentha x piperita | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.207 | (+)-isomenthone + NADPH | - |
Mentha x piperita | (+)-neoisomenthol + NADP+ | - |
? | |
1.1.1.207 | (-)-menthone + NADPH | - |
Mentha x piperita | (3R)-menthol + NADP+ | - |
? | |
1.1.1.207 | (-)-menthone + NADPH | - |
Mentha x piperita | (3R)-menthol + NADP+ | (3R)-menthol is the main product, product determination by gas chromatography, overview | ir | |
1.1.1.207 | additional information | primary monoterpene biosynthesis pathway, overview | Mentha x piperita | ? | - |
? | |
1.1.1.207 | additional information | monoterpenol is no substrate | Mentha x piperita | ? | - |
? | |
1.1.1.208 | (+)-isomenthone + NADPH | low activity | Mentha x piperita | (+)-isomenthol + NADP+ | - |
? | |
1.1.1.208 | (-)-menthone + NADPH + H+ | - |
Mentha x piperita | (3S)-neomenthol + NADP+ | - |
r | |
1.1.1.208 | (-)-menthone + NADPH + H+ | low activity in the reverse reaction | Mentha x piperita | (3S)-neomenthol + NADP+ | (3S)-neomenthol is the main product, product determination by gas chromatography, overview | r | |
1.1.1.208 | additional information | primary monoterpene biosynthesis pathway, overview | Mentha x piperita | ? | - |
? | |
1.1.1.208 | additional information | monoterpenol is a poor substrate | Mentha x piperita | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.207 | ? | x * 34070, amino acid sequence calculation | Mentha x piperita |
1.1.1.208 | ? | x * 35722, amino acid sequence calculation | Mentha x piperita |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.207 | (-)-menthone:(3R)-menthol reductase | - |
Mentha x piperita |
1.1.1.207 | MMR | - |
Mentha x piperita |
1.1.1.208 | (-)-menthone:(3S)-neomenthol reductase | - |
Mentha x piperita |
1.1.1.208 | MNR | - |
Mentha x piperita |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.207 | 31 | - |
assay at | Mentha x piperita |
1.1.1.208 | 31 | - |
assay at | Mentha x piperita |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.207 | 0.6 | - |
NADPH | pH 7.0, 31°C, recombinant enzyme | Mentha x piperita | |
1.1.1.207 | 0.6 | - |
(-)-menthone | pH 7.0, 31°C, recombinant enzyme | Mentha x piperita | |
1.1.1.208 | 0.06 | - |
NADPH | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita | |
1.1.1.208 | 0.06 | - |
(-)-menthone | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.207 | 7 | - |
- |
Mentha x piperita |
1.1.1.208 | 9.3 | - |
- |
Mentha x piperita |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.207 | 4.5 | 8.5 | 50% of maximal activity at pH 4.5 and pH 8.5 | Mentha x piperita |
1.1.1.208 | 6.5 | 10.5 | 50% of maximal activity at pH 6.5 and pH 10.5 | Mentha x piperita |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.207 | NADPH | - |
Mentha x piperita | |
1.1.1.208 | NADP+ | - |
Mentha x piperita | |
1.1.1.208 | NADPH | - |
Mentha x piperita |