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Literature summary extracted from

  • Davis, E.M.; Ringer, K.L.; McConkey, M.E.; Croteau, R.
    Monoterpene metabolism. Cloning, expression, and characterization of menthone reductases from peppermint (2005), Plant Physiol., 137, 873-881.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.207 DNA and amino acid sequence determination and analysis, expression in Escherichia coli Mentha x piperita
1.1.1.208 DNA and amino acid sequence determination and analysis, expression in Escherichia coli Mentha x piperita

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.207 0.00012
-
NADPH pH 7.0, 31°C, recombinant enzyme Mentha x piperita
1.1.1.207 0.003
-
(-)-menthone pH 7.0, 31°C, recombinant enzyme Mentha x piperita
1.1.1.207 0.041
-
(+)-isomenthone pH 7.0, 31°C, recombinant enzyme Mentha x piperita
1.1.1.208 0.0054
-
NADP+ pH 9.3, 31°C, recombinant enzyme Mentha x piperita
1.1.1.208 0.01
-
NADPH pH 9.3, 31°C, recombinant enzyme Mentha x piperita
1.1.1.208 0.674
-
(-)-menthone pH 9.3, 31°C, recombinant enzyme Mentha x piperita
1.1.1.208 1
-
(+)-isomenthone above, pH 9.3, 31°C, recombinant enzyme Mentha x piperita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.207 34070
-
x * 34070, amino acid sequence calculation Mentha x piperita
1.1.1.208 35722
-
x * 35722, amino acid sequence calculation Mentha x piperita

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.207 (+)-isomenthone + NADPH Mentha x piperita
-
(+)-neoisomenthol + NADP+
-
?
1.1.1.207 (-)-menthone + NADPH Mentha x piperita
-
(3R)-menthol + NADP+
-
?
1.1.1.207 additional information Mentha x piperita primary monoterpene biosynthesis pathway, overview ?
-
?
1.1.1.208 (+)-isomenthone + NADPH Mentha x piperita low activity (+)-isomenthol + NADP+
-
?
1.1.1.208 (-)-menthone + NADPH + H+ Mentha x piperita
-
(3S)-neomenthol + NADP+
-
r
1.1.1.208 additional information Mentha x piperita primary monoterpene biosynthesis pathway, overview ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.207 Mentha x piperita
-
cv. Black Mitchum
-
1.1.1.208 Mentha x piperita
-
cv. Black Mitchum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.207 recombinant enzyme partially Mentha x piperita
1.1.1.208 recombinant enzyme partially Mentha x piperita

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.207 leaf
-
Mentha x piperita
-
1.1.1.207 oil gland secretory cell
-
Mentha x piperita
-
1.1.1.208 leaf
-
Mentha x piperita
-
1.1.1.208 oil gland secretory cell
-
Mentha x piperita
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.207 (+)-isomenthone + NADPH
-
Mentha x piperita (+)-neoisomenthol + NADP+
-
?
1.1.1.207 (-)-menthone + NADPH
-
Mentha x piperita (3R)-menthol + NADP+
-
?
1.1.1.207 (-)-menthone + NADPH
-
Mentha x piperita (3R)-menthol + NADP+ (3R)-menthol is the main product, product determination by gas chromatography, overview ir
1.1.1.207 additional information primary monoterpene biosynthesis pathway, overview Mentha x piperita ?
-
?
1.1.1.207 additional information monoterpenol is no substrate Mentha x piperita ?
-
?
1.1.1.208 (+)-isomenthone + NADPH low activity Mentha x piperita (+)-isomenthol + NADP+
-
?
1.1.1.208 (-)-menthone + NADPH + H+
-
Mentha x piperita (3S)-neomenthol + NADP+
-
r
1.1.1.208 (-)-menthone + NADPH + H+ low activity in the reverse reaction Mentha x piperita (3S)-neomenthol + NADP+ (3S)-neomenthol is the main product, product determination by gas chromatography, overview r
1.1.1.208 additional information primary monoterpene biosynthesis pathway, overview Mentha x piperita ?
-
?
1.1.1.208 additional information monoterpenol is a poor substrate Mentha x piperita ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.207 ? x * 34070, amino acid sequence calculation Mentha x piperita
1.1.1.208 ? x * 35722, amino acid sequence calculation Mentha x piperita

Synonyms

EC Number Synonyms Comment Organism
1.1.1.207 (-)-menthone:(3R)-menthol reductase
-
Mentha x piperita
1.1.1.207 MMR
-
Mentha x piperita
1.1.1.208 (-)-menthone:(3S)-neomenthol reductase
-
Mentha x piperita
1.1.1.208 MNR
-
Mentha x piperita

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.207 31
-
assay at Mentha x piperita
1.1.1.208 31
-
assay at Mentha x piperita

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.207 0.6
-
NADPH pH 7.0, 31°C, recombinant enzyme Mentha x piperita
1.1.1.207 0.6
-
(-)-menthone pH 7.0, 31°C, recombinant enzyme Mentha x piperita
1.1.1.208 0.06
-
NADPH pH 9.3, 31°C, recombinant enzyme Mentha x piperita
1.1.1.208 0.06
-
(-)-menthone pH 9.3, 31°C, recombinant enzyme Mentha x piperita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.207 7
-
-
Mentha x piperita
1.1.1.208 9.3
-
-
Mentha x piperita

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.207 4.5 8.5 50% of maximal activity at pH 4.5 and pH 8.5 Mentha x piperita
1.1.1.208 6.5 10.5 50% of maximal activity at pH 6.5 and pH 10.5 Mentha x piperita

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.207 NADPH
-
Mentha x piperita
1.1.1.208 NADP+
-
Mentha x piperita
1.1.1.208 NADPH
-
Mentha x piperita