EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.158 | isozyme XMT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged enzyme in Escherichia coli Bl21(DE3) | Coffea arabica |
2.1.1.159 | isozyme MXMT1, DNA and amnio acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged isozymes in Escherichia coli Bl21(DE3) | Coffea arabica |
2.1.1.159 | isozyme MXMT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged isozymes in Escherichia coli Bl21(DE3) | Coffea arabica |
2.1.1.160 | isozyme DXMT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged enzyme in Escherichia coli Bl21(DE3) | Coffea arabica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.158 | 0.078 | - |
Xanthosine | recombinant XMT1 | Coffea arabica | |
2.1.1.159 | 0.012 | - |
S-adenosyl-L-methionine | recombinant MXMT1 | Coffea arabica | |
2.1.1.159 | 0.014 | - |
S-adenosyl-L-methionine | recombinant MXMT2 | Coffea arabica | |
2.1.1.159 | 0.148 | - |
7-methylxanthine | recombinant MXMT1 | Coffea arabica | |
2.1.1.159 | 0.251 | - |
7-methylxanthine | recombinant MXMT2 | Coffea arabica | |
2.1.1.159 | 0.458 | - |
paraxanthine | recombinant MXMT1 | Coffea arabica | |
2.1.1.159 | 0.738 | - |
paraxanthine | recombinant MXMT2 | Coffea arabica | |
2.1.1.160 | 0.153 | - |
S-adenosyl-L-methionine | recombinant DXMT1 | Coffea arabica | |
2.1.1.160 | 0.973 | - |
paraxanthine | recombinant DXMT1 | Coffea arabica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.158 | 41800 | - |
2 * 41800, recombinant isozyme XMT1, SDS-PAGE | Coffea arabica |
2.1.1.158 | 69000 | - |
recombinant isozyme XMT1, gel filtration | Coffea arabica |
2.1.1.159 | 43400 | - |
2 * 43400, recombinant isozyme MXMT2, SDS-PAGE | Coffea arabica |
2.1.1.159 | 74000 | - |
recombinant isozyme MXMT2, gel filtration | Coffea arabica |
2.1.1.160 | 43300 | - |
2 * 43300, recombinant isozyme DXMT1, SDS-PAGE | Coffea arabica |
2.1.1.160 | 81000 | - |
recombinant isozyme DXMT1, gel filtration | Coffea arabica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.158 | S-adenosyl-L-methionine + xanthosine | Coffea arabica | - |
S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + paraxanthine | Coffea arabica | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
2.1.1.160 | S-adenosyl-L-methionine + 3,7-dimethylxanthine | Coffea arabica | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.158 | Coffea arabica | Q9AVK0 | isozyme XMT1; isozyme XMT1 | - |
2.1.1.159 | Coffea arabica | Q84PP7 | isozyme MXMT2; isozyme MXMT2 | - |
2.1.1.159 | Coffea arabica | Q9AVJ9 | isozyme MXMT1; isozyme MXTM1 | - |
2.1.1.160 | Coffea arabica | Q8H0D2 | isozyme DXMT1; isozyme DXMT1 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.158 | flower bud | high expression | Coffea arabica | - |
2.1.1.158 | fruit | high expression level in immature fruits, low expression in mature fruits | Coffea arabica | - |
2.1.1.158 | leaf | very high expression in young leaves, moderate expression in expanded leaves | Coffea arabica | - |
2.1.1.159 | flower bud | isozyme MXMT2 | Coffea arabica | - |
2.1.1.159 | flower bud | isozyme MXTM1 | Coffea arabica | - |
2.1.1.159 | fruit | immature, isozyme MXMT2 | Coffea arabica | - |
2.1.1.159 | fruit | immature, isozyme MXTM1 | Coffea arabica | - |
2.1.1.159 | leaf | high expression in young leaves of isozyme MXTM2, low expression level in expanded leaves | Coffea arabica | - |
2.1.1.159 | leaf | very high expression in young leaves of isozyme MXTM1, low expression level in expanded leaves | Coffea arabica | - |
2.1.1.160 | flower bud | low expression level | Coffea arabica | - |
2.1.1.160 | fruit | immature | Coffea arabica | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.158 | additional information | - |
recombinant enzyme | Coffea arabica |
2.1.1.159 | additional information | - |
recombinant enzyme | Coffea arabica |
2.1.1.160 | additional information | - |
recombinant enzyme | Coffea arabica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.158 | S-adenosyl-L-methionine + xanthosine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | preferred substrate, 20fold higher activity by isozyme MXMT2 compared to activity with paraxanthine | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | preferred substrate, 3fold higher activity by isozyme MXMT1 compared to activity with paraxanthine | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + paraxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + paraxanthine | low activity by isozyme MXMT1 | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + paraxanthine | low activity by isozyme MXMT2 | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
2.1.1.160 | additional information | the multifunctional isozyme DXMT1 catalyzes also the reaction theobromine synthase, EC 2.1.1.159, but at lower activity compared to its N1-methyltransferase, i.e. caffeine synthase activity | Coffea arabica | ? | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 1,7-dimethylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 3,7-dimethylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.158 | dimer | 2 * 41800, recombinant isozyme XMT1, SDS-PAGE | Coffea arabica |
2.1.1.159 | dimer | 2 * 43400, recombinant isozyme MXMT2, SDS-PAGE | Coffea arabica |
2.1.1.160 | dimer | 2 * 43300, recombinant isozyme DXMT1, SDS-PAGE | Coffea arabica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.158 | XMT1 | - |
Coffea arabica |
2.1.1.159 | MXMT1 | - |
Coffea arabica |
2.1.1.159 | MXMT2 | - |
Coffea arabica |
2.1.1.160 | DXMT1 | - |
Coffea arabica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.158 | 27 | - |
assay at | Coffea arabica |
2.1.1.159 | 27 | - |
assay at | Coffea arabica |
2.1.1.160 | 27 | - |
assay at | Coffea arabica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.158 | 8 | - |
assay at | Coffea arabica |
2.1.1.159 | 8 | - |
assay at | Coffea arabica |
2.1.1.160 | 8 | - |
assay at | Coffea arabica |