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Literature summary extracted from

  • Wildermuth, M.C.; Fall, R.
    Biochemical characterization of stromal and thylakoid-bound isoforms of isoprene synthase in willow leaves (1998), Plant Physiol., 116, 1111-1123.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.27 diethyldicarbonate 34-59% inhibition at 1.1 mM, dimethylally diphosphate and Mg2+ protect the soluble enzyme, but not the solubilized thylakoid membrane isozyme Salix discolor
4.2.3.27 diphosphate 69-100% inhibition Salix discolor
4.2.3.27 additional information no inhibition by phosphate Salix discolor
4.2.3.27 NEM complete inactivation at 1.1 mM, dimethylally diphosphate and Mg2+ protect the isozyme partially Salix discolor
4.2.3.27 Phenylglyoxal 50 mM, complete inactivation, dimethylally diphosphate and Mg2+ protect the isozyme partially Salix discolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.27 additional information
-
 additional information soluble and membrane-bound isozymes Salix discolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.27 chloroplast soluble and membrane-bound isozymes Salix discolor 9507
-
4.2.3.27 chloroplast stroma soluble isozyme Salix discolor 9570
-
4.2.3.27 soluble soluble enzyme form Salix discolor
-
-
4.2.3.27 thylakoid membrane tightly membrane-bound isozyme with a stromal facing, membrane association via a membrane tail, accessible to trypsin treatment Salix discolor 42651
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.27 Mg2+ dependent on, isozyme activity profiles, optimal at 10-20 mM Salix discolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Salix discolor light- and age-dependent regulation of the thylakoid membrane-bound enzyme, the relation of soluble and membrane-bound isozymes is not affected by light and leaf age, overview isoprene + diphosphate isoprene is a volatile product emmitted to the atmosphere ir

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Salix discolor
-
 soluble and membrane-bound isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.27 separation of soluble and membrane-bound isozymes, by ammonium sulfate fractionation, chloroplast preparation, PEG precipitation, hydrophobic interaction and ion exchange chromatography, and gel filtration, the thylakoid membrane isozyme is irreversibly solubilized by pH 10.0 treatment, overview Salix discolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.27 leaf
-
 Salix discolor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.27 0.001
-
 purified thylakoid membrane-bound isozyme Salix discolor
4.2.3.27 0.011
-
 purified soluble isozyme Salix discolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
 Salix discolor isoprene + diphosphate i.e. 2-methyl-1,3-butadiene ir
4.2.3.27 dimethylallyl diphosphate light- and age-dependent regulation of the thylakoid membrane-bound enzyme, the relation of soluble and membrane-bound isozymes is not affected by light and leaf age, overview Salix discolor isoprene + diphosphate isoprene is a volatile product emmitted to the atmosphere ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.27 35
-
 assay at Salix discolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.27 8
-
 soluble isozyme Salix discolor
4.2.3.27 10
-
 thylakoid membrane-bound isozyme Salix discolor