BRENDA - Enzyme Database

Assessment of the effect of phosphorylated metabolites of anti-human immunodeficiency virus and anti-hepatitis B virus pyrimidine analogs on the behavior of human deoxycytidylate deaminase

Liou, J.Y.; Krishnan, P.; Hsieh, C.C.; Dutschman, G.E.; Cheng, Y.C.; Mol. Pharmacol. 63, 105-110 (2003)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
2',3'-dihydro-2',3'-dideoxy-thymidine monophosphate
-
Homo sapiens
3.5.4.12
3'-azido-2',3'-dideoxy-thymidine monophosphate
-
Homo sapiens
3.5.4.12
5-fluoro-dUMP
-
Homo sapiens
3.5.4.12
beta-D-2',2'-difluorodeoxycytidine
competitive
Homo sapiens
3.5.4.12
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
slight inhibition
Homo sapiens
3.5.4.12
beta-L-2',3'-dideoxy-5'-fluoro-3'-thiacytidine monophosphate
-
Homo sapiens
3.5.4.12
D-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
no inhibition by L-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
Homo sapiens
3.5.4.12
dUMP
product inhibition
Homo sapiens
3.5.4.12
additional information
inhibitory potency of pyrimidine phosphate analogues, overview
Homo sapiens
3.5.4.12
TMP
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.022
-
dCMP
pH 7.5
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
the enzyme is important in the de novo synthesis of thymidine nucleotides, and may also be involved in the metabolism of anticancer agents
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
native enzyme 60fold from Hep-G2 cells by adsorption chromatography
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
Hep-G2 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
beta-D-2',2'-difluorodeoxycytidine + H2O
-
670328
Homo sapiens
beta-D-2',2'-difluorodeoxyuridine + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
670328
Homo sapiens
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
the enzyme is important in the de novo synthesis of thymidine nucleotides, and may also be involved in the metabolism of anticancer agents
670328
Homo sapiens
dUMP + NH3
-
-
-
?
3.5.4.12
additional information
substrate specificity with deoxycytidine analogue monophosphates, overview
670328
Homo sapiens
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
-
assay at
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.4.12
0.0046
-
TMP
pH 7.5
Homo sapiens
3.5.4.12
0.0103
-
D-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
pH 7.5
Homo sapiens
3.5.4.12
0.0142
-
5-fluoro-dUMP
pH 7.5
Homo sapiens
3.5.4.12
0.0257
-
dUMP
pH 7.5
Homo sapiens
3.5.4.12
0.0367
-
3'-azido-2',3'-dideoxy-thymidine monophosphate
pH 7.5
Homo sapiens
3.5.4.12
0.0394
-
2',3'-dihydro-2',3'-dideoxy-thymidine monophosphate
pH 7.5
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
2',3'-dihydro-2',3'-dideoxy-thymidine monophosphate
-
Homo sapiens
3.5.4.12
3'-azido-2',3'-dideoxy-thymidine monophosphate
-
Homo sapiens
3.5.4.12
5-fluoro-dUMP
-
Homo sapiens
3.5.4.12
beta-D-2',2'-difluorodeoxycytidine
competitive
Homo sapiens
3.5.4.12
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
slight inhibition
Homo sapiens
3.5.4.12
beta-L-2',3'-dideoxy-5'-fluoro-3'-thiacytidine monophosphate
-
Homo sapiens
3.5.4.12
D-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
no inhibition by L-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
Homo sapiens
3.5.4.12
dUMP
product inhibition
Homo sapiens
3.5.4.12
additional information
inhibitory potency of pyrimidine phosphate analogues, overview
Homo sapiens
3.5.4.12
TMP
-
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.4.12
0.0046
-
TMP
pH 7.5
Homo sapiens
3.5.4.12
0.0103
-
D-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
pH 7.5
Homo sapiens
3.5.4.12
0.0142
-
5-fluoro-dUMP
pH 7.5
Homo sapiens
3.5.4.12
0.0257
-
dUMP
pH 7.5
Homo sapiens
3.5.4.12
0.0367
-
3'-azido-2',3'-dideoxy-thymidine monophosphate
pH 7.5
Homo sapiens
3.5.4.12
0.0394
-
2',3'-dihydro-2',3'-dideoxy-thymidine monophosphate
pH 7.5
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.022
-
dCMP
pH 7.5
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
the enzyme is important in the de novo synthesis of thymidine nucleotides, and may also be involved in the metabolism of anticancer agents
dUMP + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
native enzyme 60fold from Hep-G2 cells by adsorption chromatography
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
Hep-G2 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
beta-D-2',2'-difluorodeoxycytidine + H2O
-
670328
Homo sapiens
beta-D-2',2'-difluorodeoxyuridine + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
670328
Homo sapiens
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
the enzyme is important in the de novo synthesis of thymidine nucleotides, and may also be involved in the metabolism of anticancer agents
670328
Homo sapiens
dUMP + NH3
-
-
-
?
3.5.4.12
additional information
substrate specificity with deoxycytidine analogue monophosphates, overview
670328
Homo sapiens
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
-
assay at
Homo sapiens