EC Number | Cloned (Comment) | Organism |
---|---|---|
6.4.1.7 | a fragment containing alpha and beta subunit genes is engineered for heterologous expression in Escherichia coli | Hydrogenobacter thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.7 | 0.788 | - |
ATP | pH 8.5, 20°C | Hydrogenobacter thermophilus | |
6.4.1.7 | 1.03 | - |
2-oxoglutarate | pH 8.5, 20°C | Hydrogenobacter thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.7 | ATP + 2-oxoglutarate + HCO3- | Hydrogenobacter thermophilus | first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 | ADP + phosphate + oxalosuccinate | - |
? | |
6.4.1.7 | ATP + 2-oxoglutarate + HCO3- | Hydrogenobacter thermophilus TK-6 / IAM 12695 | first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 | ADP + phosphate + oxalosuccinate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.7 | Hydrogenobacter thermophilus | - |
TK-6 | - |
6.4.1.7 | Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
TK-6 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.7 | recombinant | Hydrogenobacter thermophilus |
EC Number | Storage Stability | Organism |
---|---|---|
6.4.1.7 | 4°C, stable for at least 1 month | Hydrogenobacter thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.7 | ATP + 2-oxoglutarate + HCO3- | - |
Hydrogenobacter thermophilus | ADP + phosphate + oxalosuccinate | - |
? | |
6.4.1.7 | ATP + 2-oxoglutarate + HCO3- | first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 | Hydrogenobacter thermophilus | ADP + phosphate + oxalosuccinate | - |
? | |
6.4.1.7 | ATP + 2-oxoglutarate + HCO3- | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | ADP + phosphate + oxalosuccinate | - |
? | |
6.4.1.7 | ATP + 2-oxoglutarate + HCO3- | first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ADP + phosphate + oxalosuccinate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.7 | 70 | 80 | - |
Hydrogenobacter thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.7 | 90 | - |
inactivation above | Hydrogenobacter thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.7 | 30.6 | - |
2-oxoglutarate | pH 8.5, 20°C | Hydrogenobacter thermophilus | |
6.4.1.7 | 31.7 | - |
ATP | pH 8.5, 20°C | Hydrogenobacter thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.7 | 8 | - |
- |
Hydrogenobacter thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.7 | biotin | enzyme contains biotin | Hydrogenobacter thermophilus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
6.4.1.7 | Hydrogenobacter thermophilus | isoelectric focusing | - |
4.9 |