Literature summary extracted from
Gallo, C.M.; Smith, D.L.; Smith, J.S.
Nicotinamide clearance by Pnc1 directly regulates Sir2-mediated silencing and longevity (2004), Mol. Cell. Biol., 24, 1301-1312.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.5.1.19 |
additional information |
stress suppresses the inhibitory effect of nicotinamide through the induction of PNC1 expression |
Saccharomyces cerevisiae |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.5.1.19 |
gene PNC1, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
Saccharomyces cerevisiae |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.5.1.19 |
C167A |
site-directed mutagenesis, inactive mutant, no rescue the inhibition of NAD+-dependent histone deacetylase, i.e. HDAC, activity of Sir2 by nicotinamide |
Saccharomyces cerevisiae |
3.5.1.19 |
additional information |
PNC1 overexpression suppresses the inhibitory effect of exogenously added nicotinamide on silencing, life span, and Hst1-mediated transcriptional repression |
Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.5.1.19 |
nicotinamide + H2O |
Saccharomyces cerevisiae |
nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview |
nicotinate + NH3 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.5.1.19 |
Saccharomyces cerevisiae |
- |
gene PNC1 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.5.1.19 |
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.5.1.19 |
nicotinamide + H2O |
nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview |
Saccharomyces cerevisiae |
nicotinate + NH3 |
- |
? |
|
3.5.1.19 |
nicotinamide + H2O |
Pnc1 stimulates Sir2, an NAD+-dependent histone deacetylase, and prevents the accumulation of nicotinamide |
Saccharomyces cerevisiae |
nicotinate + NH3 |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.5.1.19 |
Pnc1 |
- |
Saccharomyces cerevisiae |