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Literature summary extracted from

  • Su, Y.H.; Vacquier, V.D.
    Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa (2006), Mol. Biol. Cell, 17, 114-121.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.35 catalytic domain (COOH-terminal half-containing the catalytic domain Thr540-Ile949), expression in bacteria Strongylocentrotus purpuratus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.35 3-isobutyl-1-methylxanthine IC50: 0.032 mM Strongylocentrotus purpuratus
3.1.4.35 dipyridamole IC50: 0.023 mM Strongylocentrotus purpuratus
3.1.4.35 sildenafil IC50: 0.005 mM Strongylocentrotus purpuratus
3.1.4.35 T-1032 IC50: 0.041 mM Strongylocentrotus purpuratus
3.1.4.35 zaprinast IC50: 0.24 mM Strongylocentrotus purpuratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.35 0.01
-
 3',5'-cGMP
-
 Strongylocentrotus purpuratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.35 3',5'-cGMP + H2O Strongylocentrotus purpuratus suPDE5 regulates cGMP levels in sperm, which in turn modulate sperm motility 5'-GMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.35 Strongylocentrotus purpuratus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.35 phosphoprotein suPDE5 activity is regulated by phosphorylation. In vitro dephosphorylation of suPDE5 decreases activity by 50% Strongylocentrotus purpuratus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.35
-
 Strongylocentrotus purpuratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.35 sperm flagellum
-
 Strongylocentrotus purpuratus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.35 3',5'-cGMP + H2O suPDE5 regulates cGMP levels in sperm, which in turn modulate sperm motility Strongylocentrotus purpuratus 5'-GMP
-
 ?
3.1.4.35 3',5'-cGMP + H2O specific for 3',5'-cGMP, no activity with 3',5'-cAMP Strongylocentrotus purpuratus 5'-GMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.35 suPDE5
-
 Strongylocentrotus purpuratus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.35 7 8 3fold increase in activity from pH 7 to pH 8 Strongylocentrotus purpuratus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.35 0.005
-
 IC50: 0.005 mM Strongylocentrotus purpuratus sildenafil
3.1.4.35 0.023
-
 IC50: 0.023 mM Strongylocentrotus purpuratus dipyridamole
3.1.4.35 0.032
-
 IC50: 0.032 mM Strongylocentrotus purpuratus 3-isobutyl-1-methylxanthine
3.1.4.35 0.041
-
 IC50: 0.041 mM Strongylocentrotus purpuratus T-1032
3.1.4.35 0.24
-
 IC50: 0.24 mM Strongylocentrotus purpuratus zaprinast