BRENDA - Enzyme Database show

Regulation of aspartokinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase and dihydrodipicolinate reductase in Lactobacillus plantarum

Cahyanto, M.N.; Kawasaki, H.; Nagashio, M.; Fujiyama, K.; Seki, T.; Microbiology 152, 105-112 (2006)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.2.1.11
biotechnology
development of lysine-overproducing strains
Lactobacillus plantarum
Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.3
expression in Escherichia coli
Lactobacillus plantarum
1.2.1.11
expression in Escherichia coli Hfr3000; genes are amplified from chromosomal DNA of Lactobacillus plantarum and cloned into the pCR2.1-TOPO vector for sequencing, and subcloned into the pUC18 vector for heterologous expression in Escherichia coli
Lactobacillus plantarum
2.7.2.4
into vector pCR2.1-TOPO, subcloned to pUC18 for heterologous expression of thrA1 or thrA2 in Escherichia coli Gif 102
Lactobacillus plantarum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
additional information
Thr does not inhibit homoserine dehydrogenase 1
Lactobacillus plantarum
1.1.1.3
Thr
90% inhibition of homoserine dehydrogenase 2 at 10 mM
Lactobacillus plantarum
1.2.1.11
petrosamine B
50% inhibition at 0.306 mM. Petrosamine B is a pyridoacridine alkaloid isolated from the sponge Oceanapia sp.
Lactobacillus plantarum
1.17.1.8
additional information
no regulation of enzyme by amino acids from the aspartate family
Lactobacillus plantarum
2.7.2.4
L-isoleucine
-
Lactobacillus plantarum
2.7.2.4
L-lysine
inhibits the activity of ThrA2 by 87% at 1 mM and by 96% at 10 mM, no concerted inhibition by L-lysine plus L-threonine
Lactobacillus plantarum
2.7.2.4
L-methionine
-
Lactobacillus plantarum
2.7.2.4
L-threonine
represses, reduces growth rate, no concerted inhibition by L-lysine plus L-threonine
Lactobacillus plantarum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.2.4
Mg2+
-
Lactobacillus plantarum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Lactobacillus plantarum
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Lactobacillus plantarum NCIMB 8826
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Lactobacillus plantarum
-
-
-
1.2.1.11
Lactobacillus plantarum
-
-
-
1.2.1.11
Lactobacillus plantarum NCIMB 8826
-
-
-
1.17.1.8
Lactobacillus plantarum
-
-
-
2.7.2.4
Lactobacillus plantarum
-
-
-
2.7.2.4
Lactobacillus plantarum NCIMB 8826
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.11
cell culture
highly expressed; weakly expressed
Lactobacillus plantarum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
0.215
-
gene asd1
Lactobacillus plantarum
1.2.1.11
2.149
-
gene asd2; when expressed in Escherichia coli
Lactobacillus plantarum
1.17.1.8
1.153
-
pH 7.5, 30°C, recombinant enzyme
Lactobacillus plantarum
2.7.2.4
0.00036
-
thrA1
Lactobacillus plantarum
2.7.2.4
0.00096
-
thrA2, in the presence of L-lysine
Lactobacillus plantarum
2.7.2.4
0.162
-
thrA2
Lactobacillus plantarum
2.7.2.4
0.196
-
thrA2, in the presence of L-threonine
Lactobacillus plantarum
2.7.2.4
0.289
-
thrA2, in the presence of L-isoleucine
Lactobacillus plantarum
2.7.2.4
0.29
-
thrA2, in the presence of L-methionine
Lactobacillus plantarum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NADPH + H+
-
670165
Lactobacillus plantarum
L-homoserine + NADP+
-
-
-
?
1.1.1.3
L-aspartate 4-semialdehyde + NADPH + H+
-
670165
Lactobacillus plantarum NCIMB 8826
L-homoserine + NADP+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
670165
Lactobacillus plantarum
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
670165
Lactobacillus plantarum NCIMB 8826
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
-
670165
Lactobacillus plantarum
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
-
670165
Lactobacillus plantarum NCIMB 8826
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
2.7.2.4
ATP + L-aspartate
-
670165
Lactobacillus plantarum
ADP + 4-phospho-L-aspartate
-
-
-
?
2.7.2.4
ATP + L-aspartate
-
670165
Lactobacillus plantarum NCIMB 8826
ADP + 4-phospho-L-aspartate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
30
-
activity assay
Lactobacillus plantarum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
9
-
activity assay
Lactobacillus plantarum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADPH
-
Lactobacillus plantarum
1.2.1.11
NADPH
-
Lactobacillus plantarum
1.17.1.8
NAD(P)H
-
Lactobacillus plantarum
Application (protein specific)
EC Number
Application
Commentary
Organism
1.2.1.11
biotechnology
development of lysine-overproducing strains
Lactobacillus plantarum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
expression in Escherichia coli
Lactobacillus plantarum
1.2.1.11
expression in Escherichia coli Hfr3000; genes are amplified from chromosomal DNA of Lactobacillus plantarum and cloned into the pCR2.1-TOPO vector for sequencing, and subcloned into the pUC18 vector for heterologous expression in Escherichia coli
Lactobacillus plantarum
2.7.2.4
into vector pCR2.1-TOPO, subcloned to pUC18 for heterologous expression of thrA1 or thrA2 in Escherichia coli Gif 102
Lactobacillus plantarum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADPH
-
Lactobacillus plantarum
1.2.1.11
NADPH
-
Lactobacillus plantarum
1.17.1.8
NAD(P)H
-
Lactobacillus plantarum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
additional information
Thr does not inhibit homoserine dehydrogenase 1
Lactobacillus plantarum
1.1.1.3
Thr
90% inhibition of homoserine dehydrogenase 2 at 10 mM
Lactobacillus plantarum
1.2.1.11
petrosamine B
50% inhibition at 0.306 mM. Petrosamine B is a pyridoacridine alkaloid isolated from the sponge Oceanapia sp.
Lactobacillus plantarum
1.17.1.8
additional information
no regulation of enzyme by amino acids from the aspartate family
Lactobacillus plantarum
2.7.2.4
L-isoleucine
-
Lactobacillus plantarum
2.7.2.4
L-lysine
inhibits the activity of ThrA2 by 87% at 1 mM and by 96% at 10 mM, no concerted inhibition by L-lysine plus L-threonine
Lactobacillus plantarum
2.7.2.4
L-methionine
-
Lactobacillus plantarum
2.7.2.4
L-threonine
represses, reduces growth rate, no concerted inhibition by L-lysine plus L-threonine
Lactobacillus plantarum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.2.4
Mg2+
-
Lactobacillus plantarum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Lactobacillus plantarum
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Lactobacillus plantarum NCIMB 8826
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.11
cell culture
highly expressed; weakly expressed
Lactobacillus plantarum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
0.215
-
gene asd1
Lactobacillus plantarum
1.2.1.11
2.149
-
gene asd2; when expressed in Escherichia coli
Lactobacillus plantarum
1.17.1.8
1.153
-
pH 7.5, 30°C, recombinant enzyme
Lactobacillus plantarum
2.7.2.4
0.00036
-
thrA1
Lactobacillus plantarum
2.7.2.4
0.00096
-
thrA2, in the presence of L-lysine
Lactobacillus plantarum
2.7.2.4
0.162
-
thrA2
Lactobacillus plantarum
2.7.2.4
0.196
-
thrA2, in the presence of L-threonine
Lactobacillus plantarum
2.7.2.4
0.289
-
thrA2, in the presence of L-isoleucine
Lactobacillus plantarum
2.7.2.4
0.29
-
thrA2, in the presence of L-methionine
Lactobacillus plantarum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NADPH + H+
-
670165
Lactobacillus plantarum
L-homoserine + NADP+
-
-
-
?
1.1.1.3
L-aspartate 4-semialdehyde + NADPH + H+
-
670165
Lactobacillus plantarum NCIMB 8826
L-homoserine + NADP+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
670165
Lactobacillus plantarum
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
670165
Lactobacillus plantarum NCIMB 8826
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
-
670165
Lactobacillus plantarum
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
-
670165
Lactobacillus plantarum NCIMB 8826
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
2.7.2.4
ATP + L-aspartate
-
670165
Lactobacillus plantarum
ADP + 4-phospho-L-aspartate
-
-
-
?
2.7.2.4
ATP + L-aspartate
-
670165
Lactobacillus plantarum NCIMB 8826
ADP + 4-phospho-L-aspartate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
30
-
activity assay
Lactobacillus plantarum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
9
-
activity assay
Lactobacillus plantarum