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Literature summary extracted from

  • Kohda, D.; Yokoyama, S; Miyazawa, T.
    Thermostable valyl-tRNA, isoleucyl-tRNA and methionyl-tRNA synthetases from an extreme thermophile Thermus thermophilus HB8. Protein structure and Zn2+ binding (1984), FEBS Lett., 174, 20-23.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.9 1,10-phenanthroline
-
 Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.5 Zinc 2 Zn2+ tightly bound Thermus thermophilus
6.1.1.9 Zn2+ 2 Zn2+ tightly bound Thermus thermophilus
6.1.1.10 Zn2+ 2 Zn2+ tightly bound per enzyme molecule Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.5 115000
-
 HPLC gel filtration Thermus thermophilus
6.1.1.5 129000
-
 1 * 129000, SDS-PAGE Thermus thermophilus
6.1.1.9 100000
-
 HPLC gel filtration Thermus thermophilus
6.1.1.9 108000
-
 1 * 108000, SDS-PAGE Thermus thermophilus
6.1.1.10 75000
-
 2 * 75000, SDS-PAGE Thermus thermophilus
6.1.1.10 175000
-
 PAGE under nondissociating conditions Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.5 Thermus thermophilus
-
 HB8
-
6.1.1.5 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 HB8
-
6.1.1.9 Thermus thermophilus
-
 HB8
-
6.1.1.9 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 HB8
-
6.1.1.10 Thermus thermophilus
-
 HB8
-
6.1.1.10 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 HB8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.5
-
 Thermus thermophilus
6.1.1.9
-
 Thermus thermophilus
6.1.1.10
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
 Thermus thermophilus AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal
-
 Thermus thermophilus AMP + diphosphate + L-valyl-tRNAVal
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-valyl-tRNAVal
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Thermus thermophilus AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.5 monomer 1 * 129000, SDS-PAGE Thermus thermophilus
6.1.1.9 monomer 1 * 108000, SDS-PAGE Thermus thermophilus
6.1.1.10 dimer 2 * 75000, SDS-PAGE Thermus thermophilus