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Literature summary extracted from

  • Michalska, K.; Bujacz, G.; Jaskolski, M.
    Crystal structure of plant asparaginase (2006), J. Mol. Biol., 360, 105-116.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 additional information the enzyme performs autocatalytic activation Lupinus luteus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expression of His-tagged enzyme Lupinus luteus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.1 purified recombinant enzyme, protein solution at 10 mg/ml is mixed in 2:1 ratio with a solution containing 25% w/v PEG 4000, 100 mM HEPES, pH 6.5, and 200 mM MgCl2, incubation overnight at room temperature, the precipitate is centrifuged and the supernatant is diluted 1:1 with water, was used for hanging-drop vapor diffusion, 0.003 ml solution is mixed with 0.001 ml of reservoir solution containing 20% PEG 4000, 100 mM HEPES, pH 6.5, and 200 mM MgCl2, 200 mM MgCl2, streak-seeding with nuclei, room temperature, one day, X-ray diffraction structure determination and analysis at 2.6 A resolution, structure modelling Lupinus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Na+ probably, metal binding loop structure involving Leu59, Glu60, Ile62, Phe65, Ala67, and Ile69, overview Lupinus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Lupinus luteus
-
 L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Lupinus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 recombinant His-tagged enzyme, removal of His-tag by thrombin, anion exchange chromatography and gel filtration Lupinus luteus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.1 L-asparagine + H2O = L-aspartate + NH3 active site structure involving Thr193, autocatalytic activation mechanism involving Thr193 Lupinus luteus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Lupinus luteus L-aspartate + NH3
-
 ?
3.5.1.1 additional information the enzyme performs autocatalytic activation, overview Lupinus luteus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 tetramer (alphabeta)2, a dimer of heterodimers, quarternary structure, crystal structure, overview Lupinus luteus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase
-
 Lupinus luteus