BRENDA - Enzyme Database

D-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi: molecular cloning of the enzyme gene and crystal structure of the enzyme

Ito, K.; Nakajima, Y.; Ichihara, E.; Ogawa, K.; Katayama, N.; Nakashima, K.; Yoshimoto, T.; J. Mol. Biol. 355, 722-733 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.30
efficiently expressed in Escherichia coli cells harboring pHBDH11
Pseudomonas fragi
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.30
hanging-drop vapor-diffusion method, ligand-free enzyme and enzyme-NAD+ complex, 2.0 A resolution
Pseudomonas fragi
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.30
cacodylate
competitive
Pseudomonas fragi
1.1.1.30
dimethyl malonate
-
Pseudomonas fragi
1.1.1.30
DL-2-hydroxybutyrate
-
Pseudomonas fragi
1.1.1.30
DL-lactate
-
Pseudomonas fragi
1.1.1.30
malonate
-
Pseudomonas fragi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.30
0.13
-
NAD+
-
Pseudomonas fragi
1.1.1.30
1.2
-
(R)-3-hydroxybutanoate
-
Pseudomonas fragi
1.1.1.30
7.4
-
NADP+
-
Pseudomonas fragi
1.1.1.30
388
-
L-threonine
-
Pseudomonas fragi
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.30
27000
-
x * 27000, SDS-PAGE
Pseudomonas fragi
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.30
Pseudomonas fragi
Q5KST5
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.30
recombinant
Pseudomonas fragi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.30
(R)-3-hydroxybutanoate + NAD+
-
669873
Pseudomonas fragi
acetoacetate + NADH
-
-
-
?
1.1.1.30
(R)-3-hydroxybutanoate + NADP+
activity with NADP+ is 2% of the activity with NAD+
669873
Pseudomonas fragi
acetoacetate + NADPH
-
-
-
?
1.1.1.30
L-threonine + NAD+
-
669873
Pseudomonas fragi
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.30
?
x * 27000, SDS-PAGE
Pseudomonas fragi
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.30
7
-
reduction
Pseudomonas fragi
1.1.1.30
8.5
-
dehydrogenation
Pseudomonas fragi
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.30
NAD+
-
Pseudomonas fragi
1.1.1.30
NADP+
2% of the activity with NAD+
Pseudomonas fragi
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.30
0.81
-
DL-2-hydroxybutyrate
-
Pseudomonas fragi
1.1.1.30
0.9
-
DL-lactate
-
Pseudomonas fragi
1.1.1.30
3.8
-
malonate
-
Pseudomonas fragi
1.1.1.30
5.6
-
cacodylate
-
Pseudomonas fragi
1.1.1.30
80
-
dimethyl malonate
-
Pseudomonas fragi
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.30
efficiently expressed in Escherichia coli cells harboring pHBDH11
Pseudomonas fragi
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.30
NAD+
-
Pseudomonas fragi
1.1.1.30
NADP+
2% of the activity with NAD+
Pseudomonas fragi
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.30
hanging-drop vapor-diffusion method, ligand-free enzyme and enzyme-NAD+ complex, 2.0 A resolution
Pseudomonas fragi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.30
cacodylate
competitive
Pseudomonas fragi
1.1.1.30
dimethyl malonate
-
Pseudomonas fragi
1.1.1.30
DL-2-hydroxybutyrate
-
Pseudomonas fragi
1.1.1.30
DL-lactate
-
Pseudomonas fragi
1.1.1.30
malonate
-
Pseudomonas fragi
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.30
0.81
-
DL-2-hydroxybutyrate
-
Pseudomonas fragi
1.1.1.30
0.9
-
DL-lactate
-
Pseudomonas fragi
1.1.1.30
3.8
-
malonate
-
Pseudomonas fragi
1.1.1.30
5.6
-
cacodylate
-
Pseudomonas fragi
1.1.1.30
80
-
dimethyl malonate
-
Pseudomonas fragi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.30
0.13
-
NAD+
-
Pseudomonas fragi
1.1.1.30
1.2
-
(R)-3-hydroxybutanoate
-
Pseudomonas fragi
1.1.1.30
7.4
-
NADP+
-
Pseudomonas fragi
1.1.1.30
388
-
L-threonine
-
Pseudomonas fragi
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.30
27000
-
x * 27000, SDS-PAGE
Pseudomonas fragi
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.30
recombinant
Pseudomonas fragi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.30
(R)-3-hydroxybutanoate + NAD+
-
669873
Pseudomonas fragi
acetoacetate + NADH
-
-
-
?
1.1.1.30
(R)-3-hydroxybutanoate + NADP+
activity with NADP+ is 2% of the activity with NAD+
669873
Pseudomonas fragi
acetoacetate + NADPH
-
-
-
?
1.1.1.30
L-threonine + NAD+
-
669873
Pseudomonas fragi
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.30
?
x * 27000, SDS-PAGE
Pseudomonas fragi
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.30
7
-
reduction
Pseudomonas fragi
1.1.1.30
8.5
-
dehydrogenation
Pseudomonas fragi