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Literature summary extracted from
- D-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi: molecular cloning of the enzyme gene and crystal structure of the enzyme (2006), J. Mol. Biol., 355, 722-733.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.30 | efficiently expressed in Escherichia coli cells harboring pHBDH11 | Pseudomonas fragi |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.1.1.30 | hanging-drop vapor-diffusion method, ligand-free enzyme and enzyme-NAD+ complex, 2.0 A resolution | Pseudomonas fragi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.30 | cacodylate | competitive | Pseudomonas fragi |  |
| 1.1.1.30 | dimethyl malonate | - |
Pseudomonas fragi | |
| 1.1.1.30 | DL-2-hydroxybutyrate | - |
Pseudomonas fragi | |
| 1.1.1.30 | DL-lactate | - |
Pseudomonas fragi | |
| 1.1.1.30 | malonate | - |
Pseudomonas fragi | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.30 | 0.13 | - |
NAD+ | - |
Pseudomonas fragi | |
| 1.1.1.30 | 1.2 | - |
(R)-3-hydroxybutanoate | - |
Pseudomonas fragi | |
| 1.1.1.30 | 7.4 | - |
NADP+ | - |
Pseudomonas fragi | |
| 1.1.1.30 | 388 | - |
L-threonine | - |
Pseudomonas fragi | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.30 | 27000 | - |
x * 27000, SDS-PAGE | Pseudomonas fragi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.30 | Pseudomonas fragi | Q5KST5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.30 | recombinant | Pseudomonas fragi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.30 | (R)-3-hydroxybutanoate + NAD+ | - |
Pseudomonas fragi | acetoacetate + NADH | - |
? | |
| 1.1.1.30 | (R)-3-hydroxybutanoate + NADP+ | activity with NADP+ is 2% of the activity with NAD+ | Pseudomonas fragi | acetoacetate + NADPH | - |
? | |
| 1.1.1.30 | L-threonine + NAD+ | - |
Pseudomonas fragi | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.30 | ? | x * 27000, SDS-PAGE | Pseudomonas fragi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.30 | 7 | - |
reduction | Pseudomonas fragi |
| 1.1.1.30 | 8.5 | - |
dehydrogenation | Pseudomonas fragi |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.30 | NAD+ | - |
Pseudomonas fragi | |
| 1.1.1.30 | NADP+ | 2% of the activity with NAD+ | Pseudomonas fragi | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.30 | 0.81 | - |
DL-2-hydroxybutyrate | - |
Pseudomonas fragi | |
| 1.1.1.30 | 0.9 | - |
DL-lactate | - |
Pseudomonas fragi | |
| 1.1.1.30 | 3.8 | - |
malonate | - |
Pseudomonas fragi | |
| 1.1.1.30 | 5.6 | - |
cacodylate | - |
Pseudomonas fragi | |
| 1.1.1.30 | 80 | - |
dimethyl malonate | - |
Pseudomonas fragi | |
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