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Literature summary extracted from

  • Gaofu, Q.; Jie, L.; Rongyue, C.; Xin, Y.; Dan, M.; Jie, W.; Xiangchun, S.; Qunwei, X.; Roque, R.S.; Xiuyun, Z.; Jingjing, L.
    Asparaginase display of polypeptides in the periplasm of Escherichia coli: potential rapid pepscan technique for antigen epitope mapping (2005), J. Immunol. Methods, 299, 9-19.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expression of the mutant chimeric enzyme in the periplasm of strain BL21(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.1 additional information construction of a chimeric enzyme composed of asparaginase, a tetanus toxin peptide spacer, fragment 831-854, and the foreign cholesteryl ester transfer protein C-terminal fragment, targeting to and expression in the periplasm of Escherichia coli Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.1 periplasm
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Escherichia coli
-
 L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 native wild-type and recombinant mutant chimeric enzyme from the periplasm of strain BL21(DE3) by periplasm preparation through osmotic shock, and anion exchange chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Escherichia coli L-aspartate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 More potential rapid pepscan technique for antigen epitope mapping Escherichia coli
3.5.1.1 tetramer homotetramer Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8
-
 assay at Escherichia coli