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Literature summary extracted from
- The effect of cations on the amidase activity of human tissue kallikrein: 1-linear competitive inhibition by sodium, potassium, calcium and magnesium. 2-linear mixed inhibition by aluminium (2004), J. Enzyme Inhib. Med. Chem., 19, 317-325.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.35 | Al3+ | linear mixed inhibition | Homo sapiens |  |
| 3.4.21.35 | Ca2+ | linear competitive | Homo sapiens | |
| 3.4.21.35 | K+ | linear competitive | Homo sapiens | |
| 3.4.21.35 | Na+ | linear competitive | Homo sapiens | |
| 3.5.1.4 | Al3+ | 2-linear mixed-type inhibition of the amidase activity | Homo sapiens | |
| 3.5.1.4 | Ca2+ | 1-linear competitive inhibition of the amidase activity | Homo sapiens | |
| 3.5.1.4 | K+ | 1-linear competitive inhibition of the amidase activity | Homo sapiens | |
| 3.5.1.4 | Mg2+ | 1-linear competitive inhibition of the amidase activity | Homo sapiens | |
| 3.5.1.4 | additional information | cations interact with the active site via Asp189 and the S1 pocket via residue 29, no inhibition by soybean trypsin inhibitor | Homo sapiens | |
| 3.5.1.4 | Na+ | 1-linear competitive inhibition of the amidase activity | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.4 | extracellular | urine | Homo sapiens | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.35 | Homo sapiens | - |
- |
- |
| 3.5.1.4 | Homo sapiens | - |
healthy male individuals | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | from male urine to homogeneity | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.4 | urine | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.35 | D-Val-Leu-Arg-p-nitroanilide + H2O | - |
Homo sapiens | D-Val-Leu-Arg + p-nitroaniline | - |
? | |
| 3.5.1.4 | D-Val-L-Leu-L-Arg 4-nitroanilide + H2O | - |
Homo sapiens | D-Val-L-Leu-L-Arg + 4-nitroaniline | - |
? | |
| 3.5.1.4 | additional information | the human tissue kallikrein shows amidase activity, the enzyme hydrolyzes various synthetic substrates such as Nalpha-substituted arginine and lysine derivatives as esters, amides and fluorogenic peptides, overview | Homo sapiens | ? | - |
? | |
| 3.5.1.4 | Pro-Phe-Arg benzylamide + H2O | - |
Homo sapiens | Pro-Phe-Arg + benzylamine | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.4 | K1 | - |
Homo sapiens |
| 3.5.1.4 | tissue kallikrein | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 9 | - |
assay at | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.4 | additional information | - |
additional information | inhibition kinetics | Homo sapiens | |
| 3.5.1.4 | 2 | - |
Al3+ | pH 9.0, 37°C | Homo sapiens | |
| 3.5.1.4 | 4.9 | - |
Mg2+ | pH 9.0, 37°C | Homo sapiens | |
| 3.5.1.4 | 9.8 | - |
Ca2+ | pH 9.0, 37°C | Homo sapiens | |
| 3.5.1.4 | 22 | - |
Na+ | pH 9.0, 37°C | Homo sapiens | |
| 3.5.1.4 | 67 | - |
K+ | pH 9.0, 37°C | Homo sapiens | |
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