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Literature summary extracted from

  • Celestino, S.M.; Maria de Freitas, S.; Javier Medrano, F.; Valle de Sousa, M.; Filho, E.X.
    Purification and characterization of a novel pectinase from Acrophialophora nainiana with emphasis on its physicochemical properties (2006), J. Biotechnol., 123, 33-42.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.67 2-mercaptoethanol
-
 Acrophialophora nainiana
3.2.1.67 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Acrophialophora nainiana
3.2.1.67 diethylpyrocarbonate
-
 Acrophialophora nainiana
3.2.1.67 dithiothreitol
-
 Acrophialophora nainiana
3.2.1.67 L-cysteine
-
 Acrophialophora nainiana
3.2.1.67 L-tryptophan
-
 Acrophialophora nainiana
4.2.2.9 2,2'-dithiodipyridine
-
 Acrophialophora nainiana
4.2.2.9 5,5-dithio-bis 2-nitrobenzoic acid
-
 Acrophialophora nainiana
4.2.2.9 beta-mercaptoethanol
-
 Acrophialophora nainiana
4.2.2.9 diethyl dicarbonate
-
 Acrophialophora nainiana
4.2.2.9 dithiothreitol
-
 Acrophialophora nainiana
4.2.2.9 L-cysteine
-
 Acrophialophora nainiana
4.2.2.9 L-tryptophan
-
 Acrophialophora nainiana

Application

EC Number Application Comment Organism
4.2.2.9 industry degumming and retting of fiber crops and pre-treatment of pectic waste water from fruit juice industries Acrophialophora nainiana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 1-bromo-2,5-pyrolidinedione
-
 Acrophialophora nainiana
3.2.1.67 Al3+
-
 Acrophialophora nainiana
3.2.1.67 Ca2+
-
 Acrophialophora nainiana
3.2.1.67 Cu2+
-
 Acrophialophora nainiana
3.2.1.67 Fe2+
-
 Acrophialophora nainiana
3.2.1.67 Mn2+
-
 Acrophialophora nainiana
3.2.1.67 Zn2+
-
 Acrophialophora nainiana
4.2.2.9 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide weak inhibitor Acrophialophora nainiana
4.2.2.9 Al3+ inhibitory Acrophialophora nainiana
4.2.2.9 Ca2+ inhibitory Acrophialophora nainiana
4.2.2.9 Cu2+ inhibitory Acrophialophora nainiana
4.2.2.9 Fe2+ inhibitory Acrophialophora nainiana
4.2.2.9 Mn2+ inhibitory Acrophialophora nainiana
4.2.2.9 N-bromosuccinimide complete inhibition Acrophialophora nainiana
4.2.2.9 Zn2+ inhibitory Acrophialophora nainiana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular
-
 Acrophialophora nainiana
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.9 Mg2+ no effect Acrophialophora nainiana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 30750
-
 mass spectrometry Acrophialophora nainiana
4.2.2.9 35000
-
 SDS-PAGE, mass spectrometry Acrophialophora nainiana

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Acrophialophora nainiana
-
-
-
4.2.2.9 Acrophialophora nainiana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.9 ultrafiltration and three-step chromatographic procedures, Sephacryl S-100 column, DEAE-Sepharose ion-exchange column, Sephadex G-50 column chromatography Acrophialophora nainiana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.67 cell culture
-
 Acrophialophora nainiana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 pectin + H2O
-
 Acrophialophora nainiana digalacturonate + D-galacturonic acid
-
 ?
4.2.2.9 galacturonic acid
-
 Acrophialophora nainiana ?
-
 ?
4.2.2.9 pectin
-
 Acrophialophora nainiana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.9 monomer
-
 Acrophialophora nainiana

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 PECI
-
 Acrophialophora nainiana
3.2.1.67 pectinase
-
 Acrophialophora nainiana
4.2.2.9 exo-pectate lyase
-
 Acrophialophora nainiana
4.2.2.9 exopolymethylgalacturonate lyase
-
 Acrophialophora nainiana
4.2.2.9 PECI extracellular pectinase Acrophialophora nainiana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 60
-
-
 Acrophialophora nainiana
4.2.2.9 60
-
-
 Acrophialophora nainiana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.9 30 60
-
 Acrophialophora nainiana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 50
-
 50% activity after 7 days Acrophialophora nainiana
3.2.1.67 60
-
 50% activity after 20 min Acrophialophora nainiana
3.2.1.67 70
-
 50% activity after 3 min Acrophialophora nainiana
4.2.2.9 45 70
-
 Acrophialophora nainiana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 8
-
-
 Acrophialophora nainiana
4.2.2.9 8
-
-
 Acrophialophora nainiana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.9 8 9 up to 55°C Acrophialophora nainiana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.9 5 9 at 50-55°C Acrophialophora nainiana