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Literature summary extracted from

  • Hirano, J.; Miyamoto, K.; Ohta, H.
    Purification and characterization of the alcohol dehydrogenase with a broad substrate specificity originated from 2-phenylethanol-assimilating Brevibacterium sp. KU 1309 (2005), J. Biosci. Bioeng., 100, 318-322.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 BaCl2 1 mM, 95% inhibition Brevibacterium sp.
1.1.1.1 CuCl2 1 mM, complete inhibition Brevibacterium sp.
1.1.1.1 HgCl2 1 mM, complete inhibition Brevibacterium sp.
1.1.1.1 NiCl2 1 mM, 93% inhibition Brevibacterium sp.
1.1.1.1 PbCl2 1 mM, 24% inhibition Brevibacterium sp.
1.1.1.1 PCMB 1 mM, complete inhibition Brevibacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.012
-
 benzyl alcohol
-
 Brevibacterium sp.
1.1.1.1 0.02
-
 (R)-2-phenylpropanol
-
 Brevibacterium sp.
1.1.1.1 0.025
-
 1-phenylethanol
-
 Brevibacterium sp.
1.1.1.1 0.033
-
 3-phenylpropanol
-
 Brevibacterium sp.
1.1.1.1 0.157
-
 (S)-2-phenylpropanol
-
 Brevibacterium sp.
1.1.1.1 0.26
-
 phenylacetaldehyde
-
 Brevibacterium sp.
1.1.1.1 0.864
-
 2-phenylpropionaldehyde
-
 Brevibacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 29000
-
 gel filtration Brevibacterium sp.
1.1.1.1 39000
-
 1 * 39000, SDS-PAGE Brevibacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Brevibacterium sp.
-
 KU 1309
-
1.1.1.1 Brevibacterium sp. KU 1309
-
 KU 1309
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Brevibacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 (R)-2-phenylpropanol + NAD+ 63% of the activity with 2-phenylethanol Brevibacterium sp. (R)-2-phenylpropanal + NADH + H+
-
 ?
1.1.1.1 (R)-2-phenylpropanol + NAD+ 63% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 (R)-2-phenylpropanal + NADH + H+
-
 ?
1.1.1.1 (S)-2-phenylpropanol + NAD+ 156% of the activity with 2-phenylethanol Brevibacterium sp. (S)-2-phenylpropanal + NADH + H+
-
 ?
1.1.1.1 1-butanol + NAD+ 111% of the activity with 2-phenylethanol Brevibacterium sp. butanal + NADH
-
 ?
1.1.1.1 1-butanol + NAD+ 111% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 butanal + NADH
-
 ?
1.1.1.1 1-dodecanol + NAD+ 68% of the activity with 2-phenylethanol Brevibacterium sp. dodecanal + NADH
-
 ?
1.1.1.1 1-dodecanol + NAD+ 68% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 dodecanal + NADH
-
 ?
1.1.1.1 1-octanol + NAD+ 101% of the activity with 2-phenylethanol Brevibacterium sp. octanal + NADH
-
 ?
1.1.1.1 1-octanol + NAD+ 101% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 octanal + NADH
-
 ?
1.1.1.1 1-phenylethanol + NAD+ 46% of the activity with 2-phenylethanol Brevibacterium sp. 1-phenylethanone + NADH
-
 ?
1.1.1.1 2-phenylethanol + NAD+
-
 Brevibacterium sp. phenylacetaldehyde + NADH + H+
-
 r
1.1.1.1 2-phenylpropionaldehyde + NADH + H+
-
 Brevibacterium sp. 2-phenylpropanol + NAD+
-
 r
1.1.1.1 2-propanol + NAD+ 54% of the activity with 2-phenylethanol Brevibacterium sp. 2-propanone + NADH
-
 ?
1.1.1.1 3-phenylpropanol + NAD+ 135% of the activity with 2-phenylethanol Brevibacterium sp. 3-phenylpropanal + NADH + H+
-
 ?
1.1.1.1 benzyl alcohol + NAD+ 199% of the activity with 2-phenylethanol Brevibacterium sp. benzaldehyde + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+ 76% of the activity with 2-phenylethanol Brevibacterium sp. acetaldehyde + NADH
-
 ?
1.1.1.1 ethanol + NAD+ 76% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 acetaldehyde + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 monomer 1 * 39000, SDS-PAGE Brevibacterium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 60
-
-
 Brevibacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 35
-
 30 min, no significant loss of activity Brevibacterium sp.
1.1.1.1 50
-
 30 min, 50% loss of activity Brevibacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6
-
 reduction of phenylacetaldehyde Brevibacterium sp.
1.1.1.1 10.4
-
 oxidation of 2-phenylethanol Brevibacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 5 8 pH 5.0: about 50% of maximal activity, pH 8.0: about 40% of maximal activity, reduction of phenylacetaldehyde Brevibacterium sp.
1.1.1.1 8 12 pH 8.0: about 50% of maximal activity, pH 12.0: about 50% of maximal activity, oxidation of 2-phenylethanol Brevibacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 additional information NADP+ shows less than 0.1% of the activity with NAD+ Brevibacterium sp.
1.1.1.1 NAD+
-
 Brevibacterium sp.
1.1.1.1 NADH
-
 Brevibacterium sp.