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Literature summary extracted from

  • Kunzler, D.E.; Sasso, S.; Gamper, M.; Hilvert, D.; Kast, P.
    Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection (2005), J. Biol. Chem., 280, 32827-32834.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.3.2.1 Q91N 4fold reduction in kcat-value of enzymic activity, 10fold reduction in chorismate mutase activity Pseudomonas aeruginosa
3.3.2.1 R54K 1000fold reduction in enzymic and in chorismate mutase activity Pseudomonas aeruginosa
4.2.99.21 Q91N 20fold decrease in both isochorismate pyruvate lyase and chorismate mutase activity Pseudomonas aeruginosa
4.2.99.21 R54K 100fold decrease in both isochorismate pyruvate lyase and chorismate mutase activity Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.1 0.1
-
 isochorismate mutant R54K, pH 7.5, 30°C Pseudomonas aeruginosa
3.3.2.1 0.27
-
 isochorismate mutant Q91N, pH 7.5, 30°C Pseudomonas aeruginosa
3.3.2.1 1.06
-
 isochorismate wild-type, pH 7.5, 30°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.1 Pseudomonas aeruginosa
-
 isoform PchB, possessing mainly chorismate lyase activity
-
4.2.99.21 Pseudomonas aeruginosa
-
 enzyme possesses isochorismate pyruvate lyase and weak chorismate mutase activity
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.3.2.1 isochorismate + H2O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate (1,5)-sigmatropic reaction mechanism that invokes electrostatic catalysis Pseudomonas aeruginosa
a
4.2.99.21 isochorismate = salicylate + pyruvate [1,5]-sigmatropic reaction mechanism that invokes electrostatic catalysis in analogy to the [3,3]-pericyclic rearrangement of chorismate in chorismate mutase Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.1 Chorismate promiscuous reaction Pseudomonas aeruginosa Prephenate
-
 ?
3.3.2.1 isochorismate + H2O main reaction Pseudomonas aeruginosa 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
-
 ?
4.2.99.21 isochorismate
-
 Pseudomonas aeruginosa salicylate + pyruvate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.3.2.1 IPL
-
 Pseudomonas aeruginosa
3.3.2.1 PchB
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.1 0.003
-
 chorismate mutant R54K, pH 7.5, 30°C Pseudomonas aeruginosa
3.3.2.1 0.0062
-
 chorismate mutant Q91N, pH 7.5, 30°C Pseudomonas aeruginosa
3.3.2.1 0.085
-
 chorismate wild-type, pH 7.5, 30°C Pseudomonas aeruginosa
3.3.2.1 0.1
-
 isochorismate mutant R54K, pH 7.5, 30°C Pseudomonas aeruginosa
3.3.2.1 0.27
-
 isochorismate mutant Q91N, pH 7.5, 30°C Pseudomonas aeruginosa
3.3.2.1 1.06
-
 isochorismate wild-type, pH 7.5, 30°C Pseudomonas aeruginosa
4.2.99.21 0.1
-
 isochorismate mutant R54K, pH 7.5, 30°C Pseudomonas aeruginosa
4.2.99.21 0.27
-
 isochorismate mutant Q91N, pH 7.5, 30°C Pseudomonas aeruginosa
4.2.99.21 1.06
-
 isochorismate wild-type, pH 7.5, 30°C Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.99.21 0.9
-
 isochorismate mutant R54K, pH 7.5, 30°C Pseudomonas aeruginosa
4.2.99.21 57
-
 isochorismate mutant Q91N, pH 7.5, 30°C Pseudomonas aeruginosa
4.2.99.21 1130
-
 isochorismate wild-type, pH 7.5, 30°C Pseudomonas aeruginosa