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Literature summary extracted from

  • Singh, S.; Korolev, S.; Koroleva, O.; Zarembinski, T.; Collart, F.; Joachimiak, A.; Christendat, D.
    Crystal structure of a novel shikimate dehydrogenase from Haemophilus influenzae (2005), J. Biol. Chem., 280, 17101-17108.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 phylogenetic analysis of the three paralogues YdiB, AroE, and HI0607, DNA and amino acid sequence determination and analysis of paralogue HI0607, expression of wild-type paralogue HI0607 in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in strain B834(DE3) Haemophilus influenzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.25 purified recombinant wild-type and selenomethionine-labeled paralogue HI0607, hanging drop vapour diffusion method, 0.001 ml protein solution containing 20 mg/ml protein mixed with equal volume of reservoir solution containing 0.1 M sodium acetate, pH 4.6, 2.0 M NaCl, and 10% v/v PEG 400, X-ray diffraction structure determination and analysis at 1.75 A resolution Haemophilus influenzae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.25 D103X site-directed mutagenesis of paralogue HI0607, inactive mutant Haemophilus influenzae
1.1.1.25 K67H site-directed mutagenesis of paralogue HI0607, inactive mutant Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.25 additional information no inhibition of paralogue HI0607 by EDTA Haemophilus influenzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.25 additional information paralogue HI0607 is not affected by 5 mM of Zn2+, Ca2+, Mg2+, or Mn2+ Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 quinate + NADP+ Haemophilus influenzae reversible activity of YdiB, no activity with paralogue HI0607 3-dehydroquinate + NADPH + H+
-
 r
1.1.1.25 shikimate + NADP+ Haemophilus influenzae reversible activity of YdiB and AroE, paralogue HI0607 catalyzes the oxidative reaction with low activity 3-dehydroshikimate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Haemophilus influenzae
-
 three paralogues: YdiB, AroE, and the shikimate dehydrogenase-like HI0607
-
1.1.1.282 Enterococcus faecalis
-
-
-
1.1.1.282 Enterococcus faecium
-
-
-
1.1.1.282 Escherichia coli P0A6D5
-
-
1.1.1.282 Haemophilus influenzae P44774
-
-
1.1.1.282 Lactiplantibacillus plantarum
-
-
-
1.1.1.282 Listeria monocytogenes
-
-
-
1.1.1.282 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
1.1.1.282 Shigella flexneri
-
-
-
1.1.1.282 Streptococcus pyogenes
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.25 shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+ conserved residues Asp103 and Lys67 are important for catalysis in all three paralogues, kinetic mechanism of paralogue HI0607 and substrate binding site Haemophilus influenzae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 quinate + NADP+ reversible activity of YdiB, no activity with paralogue HI0607 Haemophilus influenzae 3-dehydroquinate + NADPH + H+
-
 r
1.1.1.25 shikimate + NADP+ reversible activity of YdiB and AroE, paralogue HI0607 catalyzes the oxidative reaction with low activity Haemophilus influenzae 3-dehydroshikimate + NADPH + H+
-
 r
1.1.1.282 shikimate + NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.282 shikimate + NAD+
-
 Lactiplantibacillus plantarum 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.282 shikimate + NAD+
-
 Enterococcus faecalis 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.282 shikimate + NAD+
-
 Streptococcus pyogenes 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.282 shikimate + NAD+
-
 Enterococcus faecium 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.282 shikimate + NAD+
-
 Listeria monocytogenes 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.282 shikimate + NAD+
-
 Shigella flexneri 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.282 shikimate + NAD+
-
 Haemophilus influenzae 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.282 shikimate + NAD+
-
 Escherichia coli 3-dehydroshikimate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.25 dimer recombinant paralogue HI0607, SDS-PAGE and gel filtration Haemophilus influenzae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.25 AroE
-
 Haemophilus influenzae
1.1.1.25 HI0607
-
 Haemophilus influenzae
1.1.1.282 quinate/shikimate dehydrogenase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.282 quinate/shikimate dehydrogenase
-
 Lactiplantibacillus plantarum
1.1.1.282 quinate/shikimate dehydrogenase
-
 Enterococcus faecalis
1.1.1.282 quinate/shikimate dehydrogenase
-
 Streptococcus pyogenes
1.1.1.282 quinate/shikimate dehydrogenase
-
 Enterococcus faecium
1.1.1.282 quinate/shikimate dehydrogenase
-
 Listeria monocytogenes
1.1.1.282 quinate/shikimate dehydrogenase
-
 Shigella flexneri
1.1.1.282 quinate/shikimate dehydrogenase
-
 Haemophilus influenzae
1.1.1.282 quinate/shikimate dehydrogenase
-
 Escherichia coli
1.1.1.282 YdiB
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.282 YdiB
-
 Lactiplantibacillus plantarum
1.1.1.282 YdiB
-
 Enterococcus faecalis
1.1.1.282 YdiB
-
 Streptococcus pyogenes
1.1.1.282 YdiB
-
 Enterococcus faecium
1.1.1.282 YdiB
-
 Listeria monocytogenes
1.1.1.282 YdiB
-
 Shigella flexneri
1.1.1.282 YdiB
-
 Haemophilus influenzae
1.1.1.282 YdiB
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.25 30
-
 assay at Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 7.5 9.8 paralogue HI0607 Haemophilus influenzae
1.1.1.25 8.5
-
 assay at Haemophilus influenzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.25 additional information
-
 pH profile, paralogue HI0607 Haemophilus influenzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NADP+ cofactor binding site of paralogue HI0607 Haemophilus influenzae
1.1.1.25 NADPH
-
 Haemophilus influenzae
1.1.1.282 NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.282 NAD+
-
 Lactiplantibacillus plantarum
1.1.1.282 NAD+
-
 Streptococcus pyogenes
1.1.1.282 NAD+
-
 Enterococcus faecium
1.1.1.282 NAD+
-
 Listeria monocytogenes
1.1.1.282 NAD+
-
 Shigella flexneri
1.1.1.282 NAD+
-
 Haemophilus influenzae
1.1.1.282 NAD+
-
 Escherichia coli