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Literature summary extracted from

  • Kashiwagi, M.; Enghild, J.J.; Gendron, C.; Hughes, C.; Caterson, B.; Itoh, Y.; Nagase, H.
    Altered Proteolytic Activities of ADAMTS-4 Expressed by C-terminal Processing (2004), J. Biol. Chem., 279, 10109-10119.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.82 expressed in 293-EBNA cells Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.82 E362A inactive mutant enzyme with mutation of Glu to Ala at position 362 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.82 additional information no inhibition by tissue inhibitor of metalloproteinases 1 or tissue inhibitor of metalloproteinases 2 at 100 nM Sus scrofa
3.4.24.82 tissue inhibitor of metalloproteinases 3 20 nM, complete inhibition Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.82 36000
-
 SDS-PAGE, small amount of ADAMTS4 found in porcupine articular cartilage without interleukin-1alpha treatment Sus scrofa
3.4.24.82 40000
-
 SDS-PAGE, small amount of ADAMTS4 found in porcupine articular cartilage without interleukin-1alpha treatment Sus scrofa
3.4.24.82 46000
-
 SDS-PAGE, primary form of ADAMTS4 in the interleukine-1alpha-treated porcupine articular cartilage Sus scrofa
3.4.24.82 70000
-
 SDS-PAGE, full-length enzyme Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.82 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.82 anti-FLAG affinity chromatography and S-200 Sephacryl gel filtration Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.82 chondrocyte
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.82 additional information
-
 39.8 units/nmol protein, one unit of enzyme activity represents the release of 0.001 mg of glycosaminoglycan/min at 37°C at pH 7.5, wild type enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.82 aggrecan + H2O
-
 Sus scrofa fragments of aggrecan
-
 ?
3.4.24.82 decorin + H2O
-
 Sus scrofa ?
-
 ?
3.4.24.82 fibromodulin + H2O
-
 Sus scrofa ?
-
 ?
3.4.24.82 transferrin + H2O
-
 Sus scrofa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.82 ADAMTS-4
-
 Sus scrofa
3.4.24.82 aggrecanase 1
-
 Sus scrofa