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Literature summary extracted from

  • Ogawa, M.; Herai, Y.; Koizumi, N.; Kusano, T.; Sano, H.
    7-Methylxanthine methyltransferase of coffee plants. Gene isolation and enzymatic properties (2001), J. Biol. Chem., 276, 8213-8218.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.158 gene CaMTL3, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species Coffea arabica
2.1.1.159 gene CaMTL2, cDNA library construction, DNA and amino acid sequence determination and analysis Coffea arabica
2.1.1.159 gene CaMXMT, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species, expression of CaMXMT as GST-fusion enzyme in Escherichia coli strain JM109 Coffea arabica
2.1.1.160 gene CaMTL1, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species Coffea arabica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.159 cytoplasm
-
 Coffea arabica 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine Coffea arabica first step in caffeine biosynthesis pathway from xanthosine S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ir
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine Coffea arabica second step in caffeine biosynthesis pathway S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine Coffea arabica second step in caffeine biosynthesis pathway from xanthosine S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea arabica last step in caffeine biosynthesis pathway S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ir

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.158 Coffea arabica Q9AVK0 gene CaMTL3, enzyme XMT1; var. caturra, two different N-methyltransferases XMT and MXMT
-
2.1.1.159 Coffea arabica Q9AVJ9 gene CaMXMT, enzyme MXMT1; var. caturra
-
2.1.1.159 Coffea arabica Q9AVK1 gene CaMTL2; var. caturra
-
2.1.1.160 Coffea arabica Q9AVJ9 gene CaMTL1; var. caturra
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.159 recombinant GST-fusion enzyme from Escherichia coli strain JM109 by glutathione affinity chromatography Coffea arabica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.158 leaf young and old leaves Coffea arabica
-
2.1.1.158 root
-
 Coffea arabica
-
2.1.1.158 stem
-
 Coffea arabica
-
2.1.1.159 bud
-
 Coffea arabica
-
2.1.1.159 leaf high expression level of MXMT in young leaves, low expression level in old leaves Coffea arabica
-
2.1.1.159 leaf old and young Coffea arabica
-
2.1.1.159 additional information wide tissue distribution Coffea arabica
-
2.1.1.159 root
-
 Coffea arabica
-
2.1.1.159 root low expression level of MXMT Coffea arabica
-
2.1.1.159 stem
-
 Coffea arabica
-
2.1.1.159 stem high expression level of MXMT Coffea arabica
-
2.1.1.160 bud
-
 Coffea arabica
-
2.1.1.160 leaf old and young Coffea arabica
-
2.1.1.160 additional information wide tissue distribution Coffea arabica
-
2.1.1.160 root
-
 Coffea arabica
-
2.1.1.160 stem
-
 Coffea arabica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine
-
 Coffea arabica S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ir
2.1.1.158 S-adenosyl-L-methionine + xanthosine first step in caffeine biosynthesis pathway from xanthosine Coffea arabica S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ir
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine second step in caffeine biosynthesis pathway Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine second step in caffeine biosynthesis pathway from xanthosine Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
2.1.1.159 S-adenosyl-L-methionine + paraxanthine low activity Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ir
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ir
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine last step in caffeine biosynthesis pathway Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ir

Synonyms

EC Number Synonyms Comment Organism
2.1.1.158 7-methylxanthosine synthase 1
-
 Coffea arabica
2.1.1.158 N-methyltransferase
-
 Coffea arabica
2.1.1.158 xanthosine methyltransferase
-
 Coffea arabica
2.1.1.158 XMT1
-
 Coffea arabica
2.1.1.159 7-methylxanthine methyltransferase
-
 Coffea arabica
2.1.1.159 7-methylxanthine N-methyltransferase
-
 Coffea arabica
2.1.1.159 MXMT
-
 Coffea arabica
2.1.1.159 MXMT1
-
 Coffea arabica
2.1.1.159 N-methyltransferase
-
 Coffea arabica
2.1.1.160 caffeine synthase
-
 Coffea arabica
2.1.1.160 CS
-
 Coffea arabica
2.1.1.160 TCS1
-
 Coffea arabica