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Literature summary extracted from

  • Tanaka, Y.; Nakagawa, N.; Kuramitsu, S.; Yokoyama, S.; Masui, R.
    Novel reaction mechanism of GTP cyclohydrolase I. High-resolution X-ray crystallography of Thermus thermophilus HB8 enzyme complexed with a transition state analogue, the 8-oxoguanine derivative (2005), J. Biochem., 138, 263-275.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.16 enzyme expression in Escherichia coli strain BL21(DE3) Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.16 purified enzyme complexed with 8-oxo-GTP or 8-oxo-dGTP, and as free enzyme, hanging drop vapour diffusion method, 20°C, 0.002 ml of 13.7 mg/ml protein is mixed with 0.002 ml reservoir solution containing 0.1 M HEPES, pH 6.8, 2.0 M ammonium sulfate, 3.4% PEG 400, and 15% glycerol, X-ray diffraction structure determination and analysis at 2.0 and 1.8 A or at 2.2. A resolution, respectively Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.16 8-oxo-dGTP competitive inhibition Thermus thermophilus
3.5.4.16 8-oxo-GTP competitive inhibition, binds tightly to the enzyme with higher affinity than GTP, structure and binding network, overview Thermus thermophilus
3.5.4.16 ATP competitive inhibition Thermus thermophilus
3.5.4.16 dGTP competitive inhibition Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.16 additional information
-
 additional information steady-state kinetics Thermus thermophilus
3.5.4.16 0.0042
-
 GTP pH 8.5, 25°C, recombinant enzyme Thermus thermophilus
3.5.4.16 0.0143
-
 GDP pH 8.5, 25°C, recombinant enzyme Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.16 Zn2+ the zinc ion is bound by residues Cys108, His111, and Cys179 Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.16 GTP + H2O Thermus thermophilus
-
 formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
3.5.4.16 GTP + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.16 Thermus thermophilus Q5SH52
-
-
3.5.4.16 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SH52
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.16 recombinant enzyme from Escherichia coli strain BL21(DE3) by ion exchange chromatography Thermus thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.16 GTP + H2O = formate + 7,8-dihydroneopterin 3'-triphosphate substrate binding and reaction mechanism, transition state structure, modelling, overview Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.16 GDP + H2O
-
 Thermus thermophilus formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine diphosphate i.e. dihydroneopterin 3'-triphosphate ?
3.5.4.16 GDP + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine diphosphate i.e. dihydroneopterin 3'-triphosphate ?
3.5.4.16 GTP + H2O
-
 Thermus thermophilus formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
3.5.4.16 GTP + H2O
-
 Thermus thermophilus formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate i.e. dihydroneopterin 3'-triphosphate ?
3.5.4.16 GTP + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
3.5.4.16 GTP + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate i.e. dihydroneopterin 3'-triphosphate ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.16 GTPCH1
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.16 25
-
 assay at Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.16 0.0017
-
 GDP pH 8.5, 25°C, recombinant enzyme Thermus thermophilus
3.5.4.16 0.0035
-
 GTP pH 8.5, 25°C, recombinant enzyme Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.16 8.5
-
 assay at Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.4.16 additional information
-
 additional information inhibition kinetics Thermus thermophilus
3.5.4.16 0.0000054
-
 8-oxo-GTP pH 8.5, 25°C, recombinant enzyme Thermus thermophilus
3.5.4.16 0.00022
-
 8-oxo-dGTP pH 8.5, 25°C, recombinant enzyme Thermus thermophilus
3.5.4.16 0.02
-
 dGTP pH 8.5, 25°C, recombinant enzyme Thermus thermophilus
3.5.4.16 0.071
-
 ATP pH 8.5, 25°C, recombinant enzyme Thermus thermophilus